Klagsbrun M, Sasse J, Sullivan R, Smith J A
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2448-52. doi: 10.1073/pnas.83.8.2448.
A human hepatoma cell line synthesizes, as evidenced by metabolic labeling, an endothelial cell mitogen that is found to be mostly cell associated. The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. HDGF is structurally related to basic fibroblast growth factor (FGF). Immunological analysis demonstrates that antiserum prepared against a synthetic peptide corresponding to the amino-terminal sequence of basic FGF cross-reacts with HDGF when analyzed by electrophoretic blotting and by immunoprecipitation. Sequence analysis of tryptic fragments demonstrates that HDGF contains sequences that are homologous to both amino-terminal and carboxyl-terminal sequences of basic FGF.
通过代谢标记证明，一种人肝癌细胞系能合成一种内皮细胞促分裂原，该促分裂原大多与细胞相关。肝癌衍生生长因子（HDGF）通过Bio-Rex 70、肝素-琼脂糖和反相色谱法相结合已被纯化至同质；它是一种阳离子多肽，分子量约为18,500 - 19,000。HDGF在结构上与碱性成纤维细胞生长因子（FGF）相关。免疫分析表明，当通过电泳印迹和免疫沉淀分析时，针对与碱性FGF氨基末端序列相对应的合成肽制备的抗血清与HDGF发生交叉反应。胰蛋白酶片段的序列分析表明，HDGF包含与碱性FGF的氨基末端和羧基末端序列同源的序列。
