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人类肿瘤在体外的类体内生长。

In vivo-like growth of human tumors in vitro.

作者信息

Freeman A E, Hoffman R M

出版信息

Proc Natl Acad Sci U S A. 1986 Apr;83(8):2694-8. doi: 10.1073/pnas.83.8.2694.

DOI:10.1073/pnas.83.8.2694
PMID:3458228
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC323366/
Abstract

We show that diverse human tumors obtained directly from surgery or biopsy can grow at high frequency in vitro for long periods of time and still maintain many of their in vivo properties. The in vivo properties maintained in vitro include three-dimensional growth; maintenance of tissue organization and structure, including changes associated with oncogenic transformation; retention of differentiated function; tumorigenicity; and the growth of multiple types of cells from a single tumor.

摘要

我们发现，直接从手术或活检中获取的多种人类肿瘤能够在体外长时间高频生长，并且仍保持其许多体内特性。体外保持的体内特性包括三维生长；维持组织的组织学和结构，包括与致癌转化相关的变化；保留分化功能；致瘤性；以及来自单个肿瘤的多种类型细胞的生长。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/3bd984c5f8eb/pnas00312-0423-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/7cee0d9de21a/pnas00312-0421-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/431cb2a51f6a/pnas00312-0421-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/a1bb43403bb5/pnas00312-0421-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/09d2ec4d9d51/pnas00312-0421-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/db890c64e641/pnas00312-0421-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/1265b5175a54/pnas00312-0421-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/d82fc4f08b99/pnas00312-0421-g.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/1f5e2e73659c/pnas00312-0422-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/578cbd480f4b/pnas00312-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/0c856b87f3be/pnas00312-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/3bd984c5f8eb/pnas00312-0423-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/7cee0d9de21a/pnas00312-0421-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/431cb2a51f6a/pnas00312-0421-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/a1bb43403bb5/pnas00312-0421-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/09d2ec4d9d51/pnas00312-0421-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/db890c64e641/pnas00312-0421-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/1265b5175a54/pnas00312-0421-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/d82fc4f08b99/pnas00312-0421-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/5fabe8b5fcaf/pnas00312-0421-h.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/7e4fe0ac2fb8/pnas00312-0422-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/203c5b86db4d/pnas00312-0422-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/1f5e2e73659c/pnas00312-0422-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/578cbd480f4b/pnas00312-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/0c856b87f3be/pnas00312-0423-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35e4/323366/3bd984c5f8eb/pnas00312-0423-c.jpg

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本文引用的文献

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2
Fine structural identification of organoid mouse lung cells cultured on a pigskin substrate.在猪皮基质上培养的类器官小鼠肺细胞的超微结构鉴定
In Vitro. 1980 Nov;16(11):994-1006. doi: 10.1007/BF02619338.
3
Histophysiologic gradient culture of stratified epithelium.复层上皮的组织生理学梯度培养
Personalising glioblastoma medicine: explant organoid applications, challenges and future perspectives.
胶质母细胞瘤治疗的个性化：外植体类器官的应用、挑战及未来展望
Acta Neuropathol Commun. 2025 Jan 11;13(1):6. doi: 10.1186/s40478-025-01928-x.
4
Cancer organoids 2.0: modelling the complexity of the tumour immune microenvironment.癌症类器官 2.0：模拟肿瘤免疫微环境的复杂性。
Nat Rev Cancer. 2024 Aug;24(8):523-539. doi: 10.1038/s41568-024-00706-6. Epub 2024 Jul 8.
5
Patient-derived tumor organoids: a new avenue for preclinical research and precision medicine in oncology.患者来源的肿瘤类器官：肿瘤学临床前研究和精准医学的新途径。
Exp Mol Med. 2024 Jul;56(7):1531-1551. doi: 10.1038/s12276-024-01272-5. Epub 2024 Jul 1.
6
Efficacy of Recombinant Methioninase on Late-stage Patient Cancer in the Histoculture Drug Response Assay (HDRA) as a Potential Functional Biomarker of Sensitivity to Methionine-restriction Therapy in the Clinic.重组蛋氨酸酶在组织培养药物反应测定（HDRA）中对晚期癌症患者的疗效，作为临床上对蛋氨酸限制疗法敏感性的潜在功能生物标志物。
Cancer Diagn Progn. 2024 May 3;4(3):239-243. doi: 10.21873/cdp.10314. eCollection 2024 May-Jun.
7
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8
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9
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5
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6
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9
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Cancer Res. 1981 Mar;41(3):1021-7.
10
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