Gilbert D M
Proc Natl Acad Sci U S A. 1986 May;83(9):2924-8. doi: 10.1073/pnas.83.9.2924.
The timing of replication of both the somatic and oocyte-type 5S ribosomal RNA genes of Xenopus laevis was determined in cultured cells by using 5-bromodeoxyuridine labeling of DNA coupled with a retroactive synchrony technique employing the fluorescence-activated cell sorter (FACS). The somatic genes replicate very early in S phase, while the oocyte genes replicate very late. These experiments provide direct evidence for a model [Gottesfeld, J. & Bloomer, L. S. (1982) Cell 28, 781-791] in which the transcription-activating factor TFIIIA is required at the time of Xenopus 5S rRNA gene replication to facilitate transcription of these genes.
利用5-溴脱氧尿苷对DNA进行标记，并结合采用荧光激活细胞分选仪（FACS）的追溯同步技术，在培养细胞中确定了非洲爪蟾体细胞型和卵母细胞型5S核糖体RNA基因的复制时间。体细胞基因在S期很早就进行复制，而卵母细胞基因则在很晚的时候复制。这些实验为一个模型[戈特斯费尔德，J. & 布卢默，L. S.（1982年）《细胞》28卷，781 - 791页]提供了直接证据，该模型认为在非洲爪蟾5S rRNA基因复制时需要转录激活因子TFIIIA来促进这些基因的转录。
