Guinta D R, Tso J Y, Narayanswami S, Hamkalo B A, Korn L J
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5150-4. doi: 10.1073/pnas.83.14.5150.
In Xenopus somatic cells, the somatic-type 5S RNA genes replicate early in S phase, bind the transcription factor TFIIIA, and are expressed; in contrast, the late replicating oocyte-type genes do not bind TFIIIA and are transcriptionally inactive. These facts support a model in which the order of replication of the somatic-type versus the oocyte-type 5S genes causes their differential expression in somatic cells due to sequestration of TFIIIA by the early-replicating somatic genes. Here we provide further evidence for the model by showing that in one Xenopus cell line in which some oocyte-type 5S genes are translocated, some oocyte-type 5S genes replicate early and are expressed.
在非洲爪蟾的体细胞中，体细胞型5S RNA基因在S期早期复制，结合转录因子TFIIIA并得以表达；相反，晚期复制的卵母细胞型基因不结合TFIIIA，转录无活性。这些事实支持了这样一种模型，即体细胞型与卵母细胞型5S基因的复制顺序导致它们在体细胞中差异表达，这是由于早期复制的体细胞基因隔离了TFIIIA。在此，我们通过表明在一个非洲爪蟾细胞系中，一些卵母细胞型5S基因发生了易位，其中一些卵母细胞型5S基因早期复制并得以表达，为该模型提供了进一步的证据。
