Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh.
Int J Biol Macromol. 2021 Nov 30;191:646-656. doi: 10.1016/j.ijbiomac.2021.09.101. Epub 2021 Sep 25.
A lectin (designated as ARL) was purified first time from the Asparagus racemosus root with the molecular weight of 14.0 kDa containing about 4.8% carbohydrate. ARL showed hemagglutination activity in both mice and human erythrocytes that were inhibited by three complex sugars among the 26 sugars tested. ARL was thermostable that mostly preserved activity at its optimum pH 8.0. Around 48% and 52.5% human colorectal cancer (HCT-116) cells growth was inhibited by 160 μg/ml of ARL and 256 μg/ml of previously purified Geodorum densiflorum rhizome lectin (GDL). Induction of apoptosis in HCT-116 cells was confirmed by Hoechst 33342 staining, caspase inhibitors, but ROS generation was only observed for ARL. The expression level of BAX and p53 genes increased with a decrease of PARP gene expression for both lectins. The expression of FAS and FADD were increased with the decrease of WNT after treatment with GDL. ARL inhibited 68% and 26% of Ehrlich ascites carcinoma cell growth in vivo in mice after treating with 3.0 and 1.5 mg/kg/day doses for five consecutive days. ARL increased the expression level of NFκB and arrested S cell cycle phase in EAC cells, in contrast, G/M phase was arrested by ARL and GDL in HCT-116.
从天门冬属植物的根中首次纯化出一种凝集素（命名为 ARL），分子量为 14.0 kDa，含有约 4.8%的碳水化合物。ARL 在小鼠和人红细胞中均表现出血凝活性，26 种测试糖中的 3 种复合糖可抑制其活性。ARL 具有热稳定性，在最适 pH 8.0 下大部分保持活性。160 μg/ml 的 ARL 和 256 μg/ml 的先前纯化的密花舌唇兰根凝集素（GDL）可抑制 48%和 52.5%的人结直肠癌细胞（HCT-116）生长。用 Hoechst 33342 染色、半胱天冬酶抑制剂证实了 HCT-116 细胞的凋亡诱导，但仅观察到 ARL 产生 ROS。BAX 和 p53 基因的表达水平增加，同时两种凝集素的 PARP 基因表达减少。用 GDL 处理后，FAS 和 FADD 的表达增加，WNT 减少。ARL 在连续 5 天每天用 3.0 和 1.5 mg/kg 剂量处理后，可分别抑制体内小鼠艾氏腹水癌细胞生长 68%和 26%。ARL 增加了 NFκB 的表达水平，并使 EAC 细胞的 S 期细胞周期停滞，相反，ARL 和 GDL 使 HCT-116 中的 G/M 期停滞。
