Kabir Syed Rashel, Islam Md Farhadul, Alom Md Jahangir, Abu Zubair Md, Absar Nurul
Department of Biochemistry and Molecular Biology, Faculty of Science, Rajshahi University, Rajshahi-6205, Bangladesh.
Protein Pept Lett. 2012 Mar;19(3):360-8. doi: 10.2174/092986612799363154.
A lectin was purified (designated as TCSL) from the Snake guard seeds with molecular mass of 56±2 kDa containing two subunits (34±1 and 22±1 kDa.). TCSL exhibited high agglutination activity at the temperature range 30 to 70°C and did not lose its activity between pH 3.0 to 12.0. The lectin was stable in the presence of denaturants and agglutinated mouse, goat, cow, chicken and human erythrocytes. TCSL did not show antifungal activity whereas it agglutinated six pathogenic bacteria and showed less toxicity against brine shrimp nauplii with the LC50 of 261±29 μg/ml. TCSL showed 28% and 72% inhibition against Ehrlich ascites carcinoma (EAC) cells in vivo in mice when administered 1 mg/kg/day and 2 mg/kg/day (i.p.) respectively for five days. TCSL enhanced the number of macrophages remarkably in the normal mice. The lectin reduced the tumor burden to 62% of EAC cells and significantly increased the hemoglobin and RBC. Treating the EAC bearing mice with TCSL at 2 mg/Kg/day for ten days with a monitoring of 20 days decreased the total WBC towards the normal level and it increased the life span by 39%.
从蛇瓜种子中纯化出一种凝集素（命名为TCSL），分子量为56±2 kDa，含有两个亚基（34±1和22±1 kDa）。TCSL在30至70°C的温度范围内表现出高凝集活性，在pH 3.0至12.0之间不会丧失活性。该凝集素在变性剂存在下稳定，能凝集小鼠、山羊、牛、鸡和人的红细胞。TCSL没有抗真菌活性，但能凝集六种病原菌，对卤虫无节幼体的毒性较小，LC50为261±29 μg/ml。当分别以1 mg/kg/天和2 mg/kg/天（腹腔注射）的剂量给小鼠连续五天注射TCSL时，其对小鼠体内艾氏腹水癌（EAC）细胞的抑制率分别为28%和72%。TCSL能显著增加正常小鼠体内巨噬细胞的数量。该凝集素可将EAC细胞的肿瘤负荷降低至62%，并显著提高血红蛋白和红细胞水平。以2 mg/Kg/天的剂量用TCSL处理荷EAC小鼠十天，并持续监测20天，可使白细胞总数降至正常水平，并使寿命延长39%。
