Parlak Hanife Merva, Karaarslan Damla, Ide Semra, Çelik Hakan Hamdi, Karabulut Erdem, Ertan Ahmet Atila, Akalın Ferda Alev
Department of Periodontology, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Department of Physics Engineering, Hacettepe University, Ankara, Turkey.
J Oral Biosci. 2021 Dec;63(4):370-377. doi: 10.1016/j.job.2021.09.007. Epub 2021 Sep 25.
During the progression of periodontitis, the structures of the cementum and saliva are altered due to pathological changes in the environment. This study aimed to analyze the nanostructures of the cervical cementum and saliva in patients with periodontitis.
Patients with periodontitis (n = 10) and periodontally healthy controls (n = 8) were included. Single-rooted teeth with indications for extraction were obtained from individuals. The cervical-thirds of the roots were sectioned transversely to obtain 1 mm thick sections. Unstimulated whole saliva samples were collected from each individual. The nanostructures of the cementum and saliva were analyzed using small and wide-angle X-ray scattering methods.
The mean radius and distance values of the cementum nanoparticles in the periodontitis and control groups were 368 Å and 1152 Å, and 377 Å and 1186 Å, respectively. The mean radius and distance values of the saliva nanoparticles in the periodontitis and control groups were 425 Å and 1359 Å, and 468 Å and 1452 Å, respectively. More wide-angle X-ray scattering profile peaks were observed in the cementum of the controls. Similarities were observed between the 3D profiles of the cementum and the saliva nanoparticles.
According to the results of the present study, (i) the cementum and saliva nanoparticles were of similar size in periodontitis and healthy controls, (ii) the cementum was more crystalline according to the (002) crystallographic plane in controls, and (iii) the similarities in the 3D-profile of the cementum and saliva nanoparticles suggest some interactions between them in the sulcus/periodontal pocket at the nanolevel.
在牙周炎进展过程中，由于环境的病理变化，牙骨质和唾液的结构会发生改变。本研究旨在分析牙周炎患者颈部牙骨质和唾液的纳米结构。
纳入牙周炎患者（n = 10）和牙周健康对照者（n = 8）。从个体获取有拔牙指征的单根牙。将牙根的颈部三分之一横向切片，获得1毫米厚的切片。从每个个体收集未刺激的全唾液样本。使用小角和广角X射线散射方法分析牙骨质和唾液的纳米结构。
牙周炎组和对照组牙骨质纳米颗粒的平均半径和间距值分别为368 Å和1152 Å，以及377 Å和1186 Å。牙周炎组和对照组唾液纳米颗粒的平均半径和间距值分别为425 Å和1359 Å，以及468 Å和1452 Å。在对照组的牙骨质中观察到更多的广角X射线散射图谱峰。在牙骨质和唾液纳米颗粒的三维图谱之间观察到相似性。
根据本研究结果，（i）牙周炎患者和健康对照者的牙骨质和唾液纳米颗粒大小相似，（ii）对照组中牙骨质根据（002）晶面结晶度更高，（iii）牙骨质和唾液纳米颗粒三维图谱的相似性表明它们在龈沟/牙周袋纳米水平存在一些相互作用。
