Bayliss O B, Adams C W
Histochem J. 1979 Jan;11(1):111-6. doi: 10.1007/BF01041270.
The aldehyde-reducing capacity of borohydride has been investigated in the sequence periodic acid-borohydride-periodic acid-Schiff and variants. Densitometric studies on rat colonic mucins show that borohydride incompletely blocks periodate-engendered aldehydes unless the pH is above 8.2. Below this value, some aldehydes are not reduced and continue to be Schiff-stainable, while others are subsequently gerenated by the second exposure to periodic acid. The effect is more pronounced in paraffin than in cryostat sections, but does not apply to human colonic mucins.
在高碘酸-硼氢化物-高碘酸-席夫序列及其变体中，对硼氢化物的醛还原能力进行了研究。对大鼠结肠粘蛋白的光密度测定研究表明，除非pH值高于8.2，硼氢化物不能完全阻断高碘酸盐产生的醛。低于这个值时，一些醛不会被还原，仍然可以被席夫染色，而其他醛随后会因再次接触高碘酸而产生。这种效应在石蜡切片中比在低温恒温器切片中更明显，但不适用于人类结肠粘蛋白。
