Reid P E, Dunn W L, Ramey C W, Coret E, Trueman L, Clay M G
Histochem J. 1984 Jun;16(6):641-9. doi: 10.1007/BF01003391.
Histochemical investigations of the periodic acid-phenylhydrazine-Schiff (PAPS) procedure were carried out on tissues containing carbohydrate macromolecules known to produce on periodate oxidation, only sialic acid monoaldehydes or hexosedialdehydes or mixtures of the two. The results indicated that the PAPS reaction is a generalized phenomenon independent of the hydrazine or hydrazide used, the nature of the Schiff reagent or the presence of anionic groups. It is proposed that phenylhydrazine condenses with periodate-engendered sialic acid monoaldehydes to yield the corresponding phenylhydrazone and with periodate-engendered dialdehydes to yield the corresponding morpholine or azido derivatives. Subsequent Schiff treatment results in the reversal of the blockade of sialic acid monoaldehydes but not of the dialdehydes, thus leading to selective Schiff staining of sialic acid residues.
对含有已知在高碘酸盐氧化时仅产生唾液酸单醛或己糖二醛或两者混合物的碳水化合物大分子的组织进行了高碘酸 - 苯肼 - 席夫（PAPS）法的组织化学研究。结果表明，PAPS反应是一种普遍现象，与所用的肼或酰肼、席夫试剂的性质或阴离子基团的存在无关。有人提出，苯肼与高碘酸盐产生的唾液酸单醛缩合生成相应的苯腙，与高碘酸盐产生的二醛缩合生成相应的吗啉或叠氮衍生物。随后的席夫处理导致唾液酸单醛的封闭被逆转，但二醛的封闭未被逆转，从而导致唾液酸残基的选择性席夫染色。
