Farhad Ali Reza, Razavi Fahimeh, Razavi Sayed Mohammad, Saatchi Masoud, Manshaei Maziar
Dental Research Center, Department of Endodontics, Dental Research Institute, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Endodontics, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2021 Aug 18;18:63. eCollection 2021.
Nitric oxide (NO) has several functions in bone healing and affects bone metabolism. Selective inducible NO synthase (iNOS) inhibitors can be used to assess the efficacy of NO for healing of bone defects. This study sought to assess the local effect of different concentrations of aminoguanidine hydrochloride (AG), a selective iNOS inhibitor, on bone healing in rats.
In this animal experimental study, 72 rats were divided into six groups of control, placebo, 5% AG, 10% AG, 15% AG, and 20% AG. A bone defect measuring 5 mm × 5 mm was created in the femur. The defect remained empty in the control group. In the placebo group, neutral gel was placed in the bone defect, and in the remaining four AG groups, different concentrations of AG were applied to the defects. Bone healing was assessed histologically. The healing score in the six groups was analyzed by the Kruskal-Wallis test. A < 0.05 was considered statistically significant.
The healing score in 20%, 15%, 10%, and 5% AG groups was significantly higher than that in the neutral gel and control groups ( < 0.01). Among the four groups of AG, 20% concentration showed better results, but the difference was not significant.
Four concentrations of AG caused greater bone healing compared to the other two groups. Selective iNOS inhibitors such as AG can be used to promote local bone healing.
一氧化氮（NO）在骨愈合中具有多种功能，并影响骨代谢。选择性诱导型一氧化氮合酶（iNOS）抑制剂可用于评估NO对骨缺损愈合的疗效。本研究旨在评估选择性iNOS抑制剂盐酸氨基胍（AG）不同浓度对大鼠骨愈合的局部影响。
在本动物实验研究中，72只大鼠被分为六组：对照组、安慰剂组、5%AG组、10%AG组、15%AG组和20%AG组。在股骨上制造一个5mm×5mm的骨缺损。对照组的缺损保持为空。在安慰剂组中，将中性凝胶置于骨缺损处，在其余四个AG组中，将不同浓度的AG应用于缺损处。通过组织学评估骨愈合情况。采用Kruskal-Wallis检验分析六组的愈合评分。P<0.05被认为具有统计学意义。
20%、15%、10%和5%AG组的愈合评分显著高于中性凝胶组和对照组（P<0.01）。在四组AG中，20%浓度组显示出更好的结果，但差异不显著。
与其他两组相比，四种浓度的AG均能促进更好的骨愈合。选择性iNOS抑制剂如AG可用于促进局部骨愈合。
