Farhad Ali Reza, Razavi Sayed Mohammad, Rozati Ali Reza, Shekarchizade Neda, Manshaei Maziar
Department of Endodontics, Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Oral Pathology, Implant Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2017 Sep-Oct;14(5):306-313. doi: 10.4103/1735-3327.215965.
Nitric oxide (NO) has many functions in wound healing and bone metabolism. This study sought to assess the local effect of aminoguanidine (AG), a selective inducible NO synthase (iNOS) inhibitor, on the rate of bone healing.
This experimental interventional study was conducted on 36 rats, which were randomly divided into three groups of control, placebo, and AG. Bone defects measuring 5 mm × 5 mm were created in the femur. In control group, bone defects remained empty. A placebo gel was applied to defects in the placebo group. AG gel was placed in bone defects in AG group. New bone formation and healing were assessed using histological and histomorphometric analyses. The healing score and the percentage of new bone formation (total bone mass, immature bone, and mature bone) were compared among the three groups using the Kruskal-Wallis test and analysis of variance, respectively. A < 0.05 was statistically significant.
The mean healing score in AG group (3.17 ± 0.577) was significantly higher than that in control (2.67 ± 0.49) and the placebo (2.58 ± 0.515) groups ( = 0.036). The percentage of new mature (lamellar) bone in AG group (22.06 ± 1.90) was significantly higher than that in control (20.94 ± 2.03) and the placebo (20.53 ± 1.20) groups ( = 0.008).
The rate of bone healing was faster in the AG compared to the other two groups. Local application of selective iNOS inhibitors like AG may be efficient as an adjunct in the clinical setting where local bone formation is required.
一氧化氮（NO）在伤口愈合和骨代谢中具有多种功能。本研究旨在评估选择性诱导型一氧化氮合酶（iNOS）抑制剂氨基胍（AG）对骨愈合速率的局部影响。
本实验性干预研究对36只大鼠进行，将其随机分为对照组、安慰剂组和AG组。在股骨上制造5毫米×5毫米的骨缺损。对照组的骨缺损保持为空。安慰剂组的缺损处应用安慰剂凝胶。AG组的骨缺损处放置AG凝胶。使用组织学和组织形态计量学分析评估新骨形成和愈合情况。分别采用Kruskal-Wallis检验和方差分析比较三组的愈合评分和新骨形成百分比（总骨量、未成熟骨和成熟骨）。P < 0.05具有统计学意义。
AG组的平均愈合评分（3.17±0.577）显著高于对照组（2.67±0.49）和安慰剂组（2.58±0.515）（P = 0.036）。AG组新成熟（板层状）骨的百分比（22.06±1.90）显著高于对照组（20.94±2.03）和安慰剂组（20.53±1.20）（P = 0.008）。
与其他两组相比，AG组的骨愈合速率更快。局部应用像AG这样的选择性iNOS抑制剂在需要局部骨形成的临床环境中作为辅助治疗可能是有效的。
