Benade L E, Talbot N, Tagliaferri P, Hardy C, Card J, Noda M, Najam N, Bassin R H
Biochem Biophys Res Commun. 1986 Apr 29;136(2):807-14. doi: 10.1016/0006-291x(86)90512-7.
Mouse cells transformed by the retroviral oncogene v-Ki- ras are significantly more sensitive to the toxic effects of 1mM ouabain than are their nontransformed counterparts. We have extended these findings to a human cell line (HOS). HOS cells (ATCC CRL 1543) are relatively resistant to treatment with 1 microM ouabain while KHOS cells (transformed by Kirsten murine sarcoma virus) are extremely sensitive. Two flat revertant cell lines isolated from the KHOS line and lacking the v- ras gene sequences are resistant to ouabain. This effect may be observed morphologically and can also be demonstrated by dye exclusion and plating efficiency tests. In addition, the toxic effects of ouabain may be rapidly and efficiently quantitated in a 51Cr-release assay. This differential lethality may be used to enrich the proportion of non-transformed revertants in populations of mutagen-treated transformed cells.
由逆转录病毒癌基因v-Ki- ras转化的小鼠细胞对1mM哇巴因的毒性作用比其未转化的对应细胞敏感得多。我们已将这些发现扩展到一种人类细胞系（HOS）。HOS细胞（ATCC CRL 1543）对1 microM哇巴因的处理相对耐受，而KHOS细胞（由 Kirsten 小鼠肉瘤病毒转化）则极其敏感。从KHOS系分离出的两个缺乏v- ras基因序列的扁平回复细胞系对哇巴因具有抗性。这种效应可以通过形态学观察到，也可以通过染料排斥和接种效率测试来证明。此外，哇巴因的毒性作用可以在51Cr释放试验中快速有效地进行定量。这种差异致死性可用于富集诱变处理的转化细胞群体中未转化回复体的比例。
