Noda M, Selinger Z, Scolnick E M, Bassin R H
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5602-6. doi: 10.1073/pnas.80.18.5602.
Two flat revertants have been isolated from mutagen-treated populations of Kirsten murine sarcoma virus (Ki-MuSV)-transformed NIH/3T3 cells. These revertants, which appear to be cellular variants resistant to transformation by the Ki-MuSV oncogene v-Ki-ras, contain Ki-MuSV-specific DNA, elevated levels of the v-Ki-ras gene product p21, and rescuable transforming virus. Cell hybridization studies indicated that the revertant phenotype is dominant in hybrids between revertant cells and cells transformed by Ki-MuSV or the closely related Harvey MuSV and BALB MuSV. Analysis of hybrid cells resulting from the fusion of these revertants to cell lines transformed by other retroviruses showed that the action of certain oncogenes structurally unrelated to v-Ki-ras also could be suppressed. Thus, there appear to be functional relationships and diversities among transforming genes (oncogenes) not readily apparent from their structural characteristics.
从经诱变处理的 Kirsten 小鼠肉瘤病毒（Ki-MuSV）转化的 NIH/3T3 细胞群体中分离出了两个扁平回复突变体。这些回复突变体似乎是对 Ki-MuSV 癌基因 v-Ki-ras 转化具有抗性的细胞变体，它们含有 Ki-MuSV 特异性 DNA、v-Ki-ras 基因产物 p21 的高水平表达以及可拯救的转化病毒。细胞杂交研究表明，回复突变体表型在回复突变体细胞与由 Ki-MuSV 或密切相关的 Harvey MuSV 和 BALB MuSV 转化的细胞之间的杂交体中占主导地位。对这些回复突变体与由其他逆转录病毒转化的细胞系融合产生的杂交细胞的分析表明，某些与 v-Ki-ras 结构不相关的癌基因的作用也可能被抑制。因此，在转化基因（癌基因）之间似乎存在功能关系和多样性，而这些从它们的结构特征中并不容易看出。
