Frassoni F, Repetto M, Podestà M, Piaggio G, Raffo M R, Sessarego M, Bacigalupo A, Marmont A M
Br J Haematol. 1986 May;63(1):135-41. doi: 10.1111/j.1365-2141.1986.tb07503.x.
Bone marrow (BM) cells from 10 patients with Ph1-positive chronic granulocytic leukaemia (CGL) were placed in long-term cultures in the presence of fetal calf serum (FCS) and horse serum (HoS), or in the presence of human AB serum. The long-term cultures were started with three different cell combinations: (1) CGL BM cells (four cases), (2) CGL BM cells + normal BM cells (1:1 ratio) from an HLA identical sex-matched sibling (five cases), (3) CGL BM cells + normal BM cells (1:1 ratio) from an HLA identical sex mismatched sibling (five cases). Cytogenetic studies were performed at weeks 0, 3, 4 and 5 of culture. The results of this study can be summarized as follows: (a) Ph1-positive cells could be detected at any time of culture in all three of the described cell combinations; (b) a population of Ph1-negative cells of patient origin could be detected after 3-5 weeks of culture; (c) there was a trend for a better survival of Ph1-negative cells in cultures supplemented with FCS + HoS and, conversely, of Ph1-positive cells in cultures containing human serum. These results warrant further studies on the possibility of manipulating survival and proliferation of normal and leukaemic cells by varying the culture conditions.
取自10例Ph1阳性慢性粒细胞白血病（CGL）患者的骨髓（BM）细胞，在胎牛血清（FCS）和马血清（HoS）存在的情况下，或在人AB血清存在的情况下进行长期培养。长期培养从三种不同的细胞组合开始：（1）CGL BM细胞（4例），（2）来自 HLA 相同的性别匹配同胞的CGL BM细胞+正常BM细胞（1:1比例）（5例），（3）来自 HLA 相同的性别不匹配同胞的CGL BM细胞+正常BM细胞（1:1比例）（5例）。在培养的第0、3、4和5周进行细胞遗传学研究。本研究结果可总结如下：（a）在所描述的所有三种细胞组合中，在培养的任何时间都能检测到Ph1阳性细胞；（b）培养3-5周后可检测到患者来源的Ph1阴性细胞群体；（c）在补充FCS+HoS的培养物中，Ph1阴性细胞有更好的存活趋势，相反，在含人血清的培养物中，Ph1阳性细胞有更好的存活趋势。这些结果值得进一步研究通过改变培养条件来控制正常细胞和白血病细胞存活及增殖的可能性。
