Homeida A M
Br J Pharmacol. 1986 Apr;87(4):673-6. doi: 10.1111/j.1476-5381.1986.tb14584.x.
Purified acetic acid extracts of corpora lutea (CL) of non-pregnant goats were found to contain substantial amounts of oxytocin (OT) as measured by radioimmunoassay. OT standard and the CL extracts released prostaglandin F2 alpha (PGF2 alpha) from rat isolated uteri in a quantitatively similar manner. Treatment of both OT standard and CL extract with sodium thioglycolate, oxytocin antiserum or oxytocin antagonist abolished this biological activity. Acid extracts of CL of pregnant goat were found to contain approximately 2% of levels during the cycle by day 21 after fertile mating and this had a reduced ability to release PGF2 alpha from rat uterus. It is concluded that both the immunoreactivity and the biological activity of the CL extracts are due to the presence of an oxytocin-like substance and that tissue levels of oxytocin are low in pregnant compared to non-pregnant goats.
通过放射免疫测定法发现，未怀孕山羊黄体（CL）的纯化乙酸提取物含有大量的催产素（OT）。OT标准品和CL提取物以定量相似的方式从大鼠离体子宫中释放前列腺素F2α（PGF2α）。用巯基乙酸钠、催产素抗血清或催产素拮抗剂处理OT标准品和CL提取物，均可消除这种生物活性。发现怀孕山羊CL的酸提取物在受孕交配后第21天所含水平约为周期中的2%，且其从大鼠子宫释放PGF2α的能力降低。得出的结论是，CL提取物的免疫反应性和生物活性均归因于一种类催产素物质的存在，并且与未怀孕山羊相比，怀孕山羊体内催产素的组织水平较低。
