Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Integrative Molecular Biomedical Sciences Graduate Program, Baylor College of Medicine, Houston, TX 77030, USA.
STAR Protoc. 2021 Sep 14;2(3):100783. doi: 10.1016/j.xpro.2021.100783. eCollection 2021 Sep 17.
We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate the need to visually screen progeny. Before using these markers in new genetic backgrounds, effective selection/counterselection concentrations should be established for each marker/drug combination. This protocol describes how to set up, perform, and analyze a drug titration curve to determine the effective selection/counterselection drug concentrations for their corresponding markers. For complete details on the use and execution of this protocol, please refer to Matinyan et al., 2021.
我们最近在果蝇遗传学中整合了一套四个选择和两个反选择标记及其相应的药物，可以单独或组合使用。这些标记消除了对后代进行视觉筛选的需要。在将这些标记用于新的遗传背景之前，应针对每个标记/药物组合建立有效的选择/反选择浓度。本方案描述了如何建立、执行和分析药物滴定曲线，以确定相应标记的有效选择/反选择药物浓度。有关此方案的使用和执行的完整详细信息，请参阅 Matinyan 等人，2021。
