Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, China.
STAR Protoc. 2021 Sep 17;2(4):100814. doi: 10.1016/j.xpro.2021.100814. eCollection 2021 Dec 17.
N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by , causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This model can be widely used for studying the initiation and growth of gastric cancer. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
N-甲基-N-亚硝脲，一种由 转化而来的亚硝胺化合物，可引起上皮细胞的体细胞突变，并诱导胃前恶性病变。在这里，我们描述了一种在小鼠中诱导胃肿瘤和分析肿瘤表型的详细方案。该模型可广泛用于研究胃癌的发生和发展。有关该方案使用和执行的完整详细信息，请参阅 Li 等人（2021 年）。
