Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, China.
Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, China.
J Fish Dis. 2021 Dec;44(12):2111-2123. doi: 10.1111/jfd.13524. Epub 2021 Sep 28.
Amyloodinium ocellatum (AO) infection in silver pomfret (Pampus argenteus) causes extensive mortality. Insufficient information exists on the molecular immune response of silver pomfret to AO infestation, so herein we simulated the process of silver pomfret being infected by AO. Translucent trophosomes were observed on the gills of AO-infected fish. Transcriptome profiling was performed to investigate the effects of AO infection on the gill, kidney complex and spleen. Overall, 404,412,298 clean reads were obtained, assembling into 96,341 unigenes, which were annotated against public databases. In total, 2730 differentially expressed genes were detected, and few energy- and immune-related genes were further assessed using RT-qPCR. Moreover, activities of three immune-related (SOD, AKP and ACP) and three energy-related (PKM, LDH and GCK) enzymes were determined. AO infection activated the immune system and increased interleukin-1 beta and immunoglobulin M heavy chain levels. Besides, the PPAR signalling pathway was highly enriched, which played a role in improving immunity and maintaining homeostasis. AO infection also caused dyspnoea, leading to extensive lactic acid accumulation, potentially contributing towards a strong immune response in the host. Our data improved our understanding regarding the immune response mechanisms through which fish coped with parasitic infections and may help prevent high fish mortality in aquaculture.
眼斑拟微绿球藻（AO）感染银鲳（Pampus argenteus）会导致大量死亡。关于银鲳受到 AO 侵袭后的分子免疫反应，目前的信息还不够充分，因此，我们在这里模拟了银鲳被 AO 感染的过程。在 AO 感染的鱼的鳃上观察到半透明的滋养体。进行转录组谱分析，以研究 AO 感染对鳃、肾复合器官和脾的影响。总的来说，我们获得了 404,412,298 条清洁读数，组装成 96,341 个非编码基因，并对其进行了公共数据库注释。共检测到 2730 个差异表达基因，并用 RT-qPCR 进一步评估了少数与能量和免疫相关的基因。此外，还测定了三种免疫相关（SOD、AKP 和 ACP）和三种能量相关（PKM、LDH 和 GCK）酶的活性。AO 感染激活了免疫系统，增加了白细胞介素 1β和免疫球蛋白 M 重链的水平。此外，PPAR 信号通路高度富集，在提高免疫力和维持体内平衡方面发挥作用。AO 感染还导致呼吸困难，导致大量乳酸积累，可能导致宿主产生强烈的免疫反应。我们的数据增进了我们对鱼类应对寄生虫感染的免疫反应机制的理解，可能有助于防止水产养殖中鱼类死亡率过高。
