Kuzmits R, Aiginger P, Müller M M, Steurer G, Linkesch W
Clin Sci (Lond). 1986 Jul;71(1):81-8. doi: 10.1042/cs0710081.
A short-term test in vitro is described, which can be used to detect resistance to cytostatic agents in leukaemic cells. Leukaemic cell suspensions were incubated with cytostatic agents and the resulting intracellular ATP concentrations were measured by a bioluminescence ATP assay. There was a clear dose-effect relationship in acute leukaemia and chronic lymphocytic leukaemia cells for drugs used in the treatment of leukaemias. A good correlation was found between the ATP content of leukaemic cells and cell viability as determined by the trypan blue dye exclusion test. Preliminary individual clinical correlations suggest a correlation between the chemosensitivity in vitro and the response patterns in vivo in leukaemia patients. This simple, fast and sensitive method may have application for determination of drug-induced cytotoxicity in leukaemic cells in vitro.
本文描述了一种体外短期试验，可用于检测白血病细胞对细胞抑制剂的耐药性。将白血病细胞悬液与细胞抑制剂一起孵育，然后通过生物发光ATP检测法测量所得细胞内ATP浓度。对于用于治疗白血病的药物，急性白血病和慢性淋巴细胞白血病细胞存在明显的剂量效应关系。通过台盼蓝染料排除试验测定，发现白血病细胞的ATP含量与细胞活力之间具有良好的相关性。初步的个体临床相关性表明，白血病患者的体外化学敏感性与体内反应模式之间存在相关性。这种简单、快速且灵敏的方法可能适用于体外测定白血病细胞中药物诱导的细胞毒性。
