Universidade de São Paulo, Faculdade de Medicina, Laboratório de Investigação Médica em Protozoologia, Bacteriologia e Resistência Antimicrobiana (LIM 49), São Paulo, São Paulo, Brazil.
Universidade de São Paulo, Laboratório de Virologia (LIM 52), São Paulo, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2021 Sep 27;63:e74. doi: 10.1590/S1678-9946202163074. eCollection 2021.
Mobile phones (MPs) have become an important work tool around the world including in hospitals. We evaluated whether SARS-CoV-2 can remain on the surface of MPs of first-line healthcare workers (HCW) and also the knowledge of HCWs about SARS-CoV-2 cross-transmission and conceptions on the virus survival on the MPs of HCWs. A cross-sectional study was conducted in the COVID-19 Intensive Care Unit of a teaching hospital. An educational campaign was carried out on cross-transmission of SARS-CoV-2, and its permanence in fomites, in addition to the proper use and disinfection of MPs. Herewith an electronic questionnaire was applied including queried conceptions about hand hygiene and care with MP before and after the pandemic. The MPs were swabbed with a nylon FLOQ Swab™, in an attempt to increase the recovery of SARS-CoV-2. All MP swab samples were subjected to SARS-CoV-2 RT-PCR; RT-PCR positive samples were subjected to viral culture in Vero cells (ATCC® CCL-81™). Fifty-one MPs were swabbed and a questionnaire on hand hygiene and the use and disinfection of MP was applied after an educational campaign. Most HCWs increased adherence to hand hygiene and MP disinfection during the pandemic. Fifty-one MP swabs were collected and two were positive by RT-PCR (4%), with Cycle threshold (Ct ) values of 34-36, however, the cultures of these samples were negative. Although most HCWs believed in the importance of cross-transmission and increased adherence to hand hygiene and disinfection of MP during the pandemic, SARS-CoV-2 RNA was detected in MPs. Our results suggest the need for a universal policy in infection control guidelines on how to care for electronic devices in hospital settings.
手机（MPs）已成为全球范围内的一种重要工作工具，包括在医院中。我们评估了 SARS-CoV-2 是否可以在一线医护人员（HCW）的 MPs 表面存活，以及 HCW 对 SARS-CoV-2 交叉传播的认识和对 HCW MPs 上病毒存活的观念。在一家教学医院的 COVID-19 重症监护病房进行了一项横断面研究。开展了关于 SARS-CoV-2 交叉传播及其在污染物中的持久性，以及 MPs 的正确使用和消毒的教育活动。同时，应用了电子问卷，包括在大流行前后对手卫生和 MP 护理的概念进行了查询。用尼龙 FLOQ Swab™对 MPs 进行了拭子取样，以增加 SARS-CoV-2 的回收。所有 MP 拭子样本均进行了 SARS-CoV-2 RT-PCR；RT-PCR 阳性样本在 Vero 细胞（ATCC® CCL-81™）中进行了病毒培养。对 51 个 MPs 进行了拭子取样，并在教育活动后应用了有关手卫生以及 MP 使用和消毒的问卷。大多数 HCW 在大流行期间增加了对手卫生和 MP 消毒的依从性。收集了 51 个 MPs 拭子，其中两个通过 RT-PCR 呈阳性（4%），Ct 值为 34-36，但这些样本的培养均为阴性。尽管大多数 HCW 相信交叉传播的重要性，并在大流行期间增加了对手卫生和 MP 消毒的依从性，但在 MPs 中检测到了 SARS-CoV-2 RNA。我们的结果表明，需要在医院环境中制定关于如何护理电子设备的感染控制指南的普遍政策。
