Zero D T, van Houte J, Russo J
J Dent Res. 1986 Jun;65(6):918-23. doi: 10.1177/00220345860650061201.
The role of extracellular matrix material (EMM) synthesized from sucrose (S) by Streptococcus mutans IB-1600 in altering the demineralizing potential of artificial plaque was evaluated with an intraoral enamel demineralization test (IEDT). The artificial plaque samples were prepared from cells cultivated in Todd-Hewitt broth (THB) supplemented with various S concentrations and by mixing THB-grown cells with increasing proportions of EMM (heat-killed THB + 2% S-cultivated cells). The samples were also evaluated for cell density (DNA content) and acidogenicity in vitro (pH-stat), as well as for in situ pH changes during a 45-minute intra-oral test following a 10% glucose rinse. An increase in the proportion of EMM relative to cell density was associated with an increase in enamel demineralization. This trend reversed when the ratio of cells to EMM was less than 1:19. Experiments involving strains of S. mitis, S. sanguis, and S. salivarius suggested a similar effect of EMM. The intra-oral pH data suggest that the presence of EMM may enhance demineralization by altering diffusion properties of plaque.
通过口腔内釉质脱矿试验（IEDT）评估了变形链球菌IB - 1600利用蔗糖（S）合成的细胞外基质材料（EMM）在改变人工菌斑脱矿潜力方面的作用。人工菌斑样本由在添加不同浓度S的托德 - 休伊特肉汤（THB）中培养的细胞制备而成，以及通过将在THB中生长的细胞与比例不断增加的EMM（热灭活的THB + 2% S培养细胞）混合制备而成。还对样本进行了细胞密度（DNA含量）和体外产酸性（pH计）评估，以及在10%葡萄糖漱口后的45分钟口腔内测试期间的原位pH变化评估。相对于细胞密度，EMM比例的增加与釉质脱矿增加相关。当细胞与EMM的比例小于1:19时，这种趋势逆转。涉及缓症链球菌、血链球菌和唾液链球菌菌株的实验表明EMM有类似作用。口腔内pH数据表明，EMM的存在可能通过改变菌斑的扩散特性来增强脱矿作用。
