University Clinical Centre Maribor, Department of Psychiatry, Maribor, Slovenia.
University Psychiatric Clinic, Ljubljana, Slovenia.
Arh Hig Rada Toksikol. 2021 Sep 28;72(3):191-197. doi: 10.2478/aiht-2021-72-3432.
We investigated two functional polymorphisms in NLRP3 inflammasome genes ( rs35829419 and rs2043211) and their association with alcohol dependence and related anxiety, depression, obsession-compulsion, or aggression in 88 hospitalised alcohol-dependent patients, 99 abstinent alcohol-dependent participants, and 94 controls, all male Caucasian. Alcohol dependence-related psychiatric disorders were assessed with the Zung Depression and Anxiety scale, Buss-Durkee Hostility Inventory, Alcohol Use Disorders Identification Test, Brief Social Phobia Scale, Obsessive Compulsive Drinking Scale, and Yale-Brown Obsessive-Compulsive Scale. For genotyping we used the allele-specific quantitative polymerase chain reaction-based methods. The three groups differed significantly in rs2043211 distribution (P=0.049; chi-squared=9.557; df=4). The rs35829419 polymorphism was not significantly associated with alcohol dependence. In hospitalised alcohol-dependent patients the investigated polymorphisms were not associated with scores indicating alcohol consumption or comorbid symptoms. In abstinent alcohol-dependent subjects homozygotes for the polymorphic allele presented with the highest scores on the Zung Anxiety Scale (p=0.048; df=2; F=3.140). Among controls, genotype was associated with high scores on the Obsessive Compulsive Drinking Scale (P=0.027; df=2; F=3.744). In conclusion, our results reveal that rs2043211 may play some role in susceptibility to alcohol dependence, expression of anxiety symptoms in abstinent alcohol-dependent subjects, and in obsessive compulsive drinking in healthy controls. However, further studies with larger cohorts are required to confirm these preliminary findings.
我们研究了 NLRP3 炎症小体基因中的两个功能多态性（rs35829419 和 rs2043211），以及它们与 88 名住院酒精依赖患者、99 名戒酒酒精依赖参与者和 94 名对照组男性白种人之间的酒精依赖和相关焦虑、抑郁、强迫-冲动或攻击的关联。使用 Zung 抑郁和焦虑量表、Buss-Durkee 敌意量表、酒精使用障碍识别测试、Brief Social Phobia Scale、Obsessive Compulsive Drinking Scale 和 Yale-Brown Obsessive-Compulsive Scale 评估与酒精依赖相关的精神疾病。使用基于等位基因特异性定量聚合酶链反应的方法进行基因分型。三组在 rs2043211 分布上差异显著（P=0.049；卡方=9.557；df=4）。rs35829419 多态性与酒精依赖无显著相关性。在住院酒精依赖患者中，所研究的多态性与表明饮酒或共病症状的评分无关。在戒酒的酒精依赖受试者中，多态性等位基因的纯合子在 Zung 焦虑量表上的得分最高（p=0.048；df=2；F=3.140）。在对照组中，基因型与 Obsessive Compulsive Drinking Scale 的高分相关（P=0.027；df=2；F=3.744）。总之，我们的结果表明，rs2043211 可能在易感性、戒酒的酒精依赖受试者中焦虑症状的表达以及健康对照组中强迫性饮酒方面起一定作用。然而，需要进一步的大样本队列研究来证实这些初步发现。
