Yi Xin Low, Hui Min Toh, Nur Liyana Mohd Zin Puteri, Pulingam Thiruchelvi, Nelson Appaturi Jimmy, Parumasivam Thaigarajan
School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Saudi J Biol Sci. 2021 Oct;28(10):5884-5889. doi: 10.1016/j.sjbs.2021.06.036. Epub 2021 Jun 19.
This study aimed to evaluate the antibacterial activities of 61 plant extracts from 49 Malaysian ethnomedicinal plants and to investigate the interaction of the active plant extracts in combination with synthetic antibiotics against the MSSA and MRSA strains. The minimum inhibitory concentration MIC) and minimum bactericidal concentration (MBC) of the plant extracts were determined using a microdilution method against MSSA and MRSA strains. The interaction between active plant extracts and the antibiotics was assessed using the checkerboard method. The total fractional inhibitory concentration (∑FIC) indices from the combination were calculated to determine the nature of the interaction. Out of the 61 plant extracts tested against the MSSA strain, 7 plant extracts (~ 11%) showed MIC values of less than 200 μg/mL, 17 extracts (~ 28%) showed MIC between 200 and 800 µg/mL and seed extracts of showed MBC values of 400 μg/mL. The seed extract of showed MIC and MBC of 400 μg/mL against the MRSA strains while leaf extract of showed MIC of 400 μg/mL against MRSA NCTC 12493. When the active plant extracts (MIC ≤ 200 µg/mL for MSSA, and ≤ 400 µg/mL for MRSA) were tested in combination with vancomycin and ciprofloxacin, they showed no interaction against both MSSA and MRSA with ∑FIC between 1.06 and 2.03. These findings provide a preliminary overview of the anti-MSSA and anti-MRSA properties of Malaysian ethnobotanical plants to combat infections. Further research is needed to establish an antibacterial profile of the tested plant extracts.
本研究旨在评估来自49种马来西亚民族药用植物的61种植物提取物的抗菌活性，并研究活性植物提取物与合成抗生素联合使用对甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）菌株的相互作用。采用微量稀释法测定植物提取物对MSSA和MRSA菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。使用棋盘法评估活性植物提取物与抗生素之间的相互作用。计算联合用药的总分数抑菌浓度（∑FIC）指数，以确定相互作用的性质。在针对MSSA菌株测试的61种植物提取物中，7种植物提取物（约11%）的MIC值小于200μg/mL，17种提取物（约28%）的MIC在200至800μg/mL之间，[植物名称未给出]的种子提取物的MBC值为400μg/mL。[植物名称未给出]的种子提取物对MRSA菌株的MIC和MBC均为400μg/mL，而[植物名称未给出]的叶提取物对MRSA NCTC 12493的MIC为400μg/mL。当活性植物提取物（对MSSA的MIC≤200μg/mL，对MRSA的MIC≤400μg/mL）与万古霉素和环丙沙星联合测试时，它们对MSSA和MRSA均无相互作用，∑FIC在1.06至2.03之间。这些发现为马来西亚民族植物学植物抗MSSA和抗MRSA特性以对抗[感染类型未给出]感染提供了初步概述。需要进一步研究以确定所测试植物提取物的抗菌谱。
