Soligo D, Polli N, Cattoretti G, Cantu'-Rajnoldi A, Romitti L, De Harven E, Lambertenghi-Deliliers G
J Submicrosc Cytol. 1986 Apr;18(2):433-40.
A case of Ph1 positive acute leukaemia is presented in which an additional chromosome change, monosomy 7 was found. There was no clinical evidence of a pre-existing chronic myeloid leukaemia. Cytochemistry and immunology showed a predominant HLA-DR+, TdT+, cALL- phenotype, with a small percentage of HLA-DR+, Leu-Ml+ and cALL- cells. The true biphenotypic nature of this case was clearly shown by transmission electron microscopy using the immunogold method combined with myeloperoxidase (MPO). Two distinct phenotypes, lymphoid (cALL+, MPO-) and myeloid (LeuMl+, MPO+) were identified with this technique. An immuno-scanning electron microscope technique was also used to study this case, which demonstrated the presence of two different surface morphologies.
本文报告1例Ph1阳性急性白血病，发现其存在额外的染色体改变——7号染色体单体。无既往慢性髓性白血病的临床证据。细胞化学和免疫学检查显示主要为HLA-DR+、TdT+、cALL-表型，有一小部分HLA-DR+、Leu-Ml+和cALL-细胞。通过使用免疫金法结合髓过氧化物酶（MPO）的透射电子显微镜清楚地显示了该病例真正的双表型性质。用该技术鉴定出两种不同的表型，即淋巴样（cALL+、MPO-）和髓样（LeuMl+、MPO+）。还使用免疫扫描电子显微镜技术研究该病例，其显示存在两种不同的表面形态。
