Addis Ababa University, Department of Physics, Addis Ababa, Ethiopia.
Middle Tennessee State University, Department of Physics, Murfreesboro, Tennessee, United States.
J Biomed Opt. 2021 Sep;26(9). doi: 10.1117/1.JBO.26.9.096502.
We introduce a model for better calibration of the trapping force using an equal but oppositely directed drag force acting on a trapped red blood cell (RBC). We demonstrate this approach by studying RBCs' elastic properties from deidentified sickle cell anemia (SCA) and sickle cell trait (SCT) blood samples.
A laser trapping (LT) force was formulated and analytically calculated in a cylindrical model. Using this trapping force relative percent difference, the maximum (longitudinal) and minimum (transverse) radius rate and stiffness were used to study the elasticity.
The elastic property of SCA and SCT RBCs was analyzed using LT technique with computer controlled piezo-driven stage, in order to trap and stretch the RBCs.
For all parameters, the results show that the SCT RBC samples have higher elastic property than the SCA RBCs. The higher rigidity in the SCA cell may be due to the lipid composition of the membrane, which was affected by the cholesterol concentration.
By developing a theoretical model for different trapping forces, we have also studied the elasticity of RBCs in SCT (with hemoglobin type HbAS) and in SCA (with hemoglobin type HbSS). The results for the quantities describing the elasticity of the cells consistently showed that the RBCs in the SCT display lower rigidity and higher deformability than the RBCs with SCA.
我们引入了一种使用作用于被困红细胞 (RBC) 的相等但相反方向的阻力来更好地校准捕获力的模型。我们通过研究来自无身份识别的镰状细胞贫血 (SCA) 和镰状细胞特征 (SCT) 血液样本的 RBC 弹性特性来证明这种方法。
制定了一种激光捕获 (LT) 力的模型，并在圆柱模型中进行了分析计算。使用这种捕获力的相对百分比差异，最大（纵向）和最小（横向）半径率和刚度用于研究弹性。
使用计算机控制的压电驱动台的 LT 技术来分析 SCA 和 SCT RBC 的弹性特性，以便捕获和拉伸 RBC。
对于所有参数，结果表明 SCT RBC 样本的弹性特性高于 SCA RBC。SCA 细胞的刚性较高可能是由于膜的脂质组成受到胆固醇浓度的影响。
通过开发用于不同捕获力的理论模型，我们还研究了 SCT（血红蛋白类型为 HbAS）和 SCA（血红蛋白类型为 HbSS）中 RBC 的弹性。描述细胞弹性的量的结果一致表明，SCT 中的 RBC 比 SCA 中的 RBC 刚性更低，变形能力更高。
