Regenerative Nanomedicine Lab, Department of Chemical Engineering, University of Waterloo, Waterloo, ON, Canada.
Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.
Methods Mol Biol. 2022;2375:177-189. doi: 10.1007/978-1-0716-1708-3_15.
Preclinical testing in animal model is a required stage of vascular device development. Among small animal models, rabbits provide vasculature with relative larger caliber for anastomotic implantation of vascular grafts as preclinical testing before conducting large animal studies. Rabbits have similar hemostatic mechanism with human and can accommodate vascular grafts with various diameters at different locations, and thus provide a valid model to assess small-diameter vascular grafts. This chapter will describe the procedures and materials required to conduct survival surgery in rabbit carotid artery models for implantation of small-diameter tubular grafts with an end-to-side and end-to-end anastomotic technique.
动物模型的临床前测试是血管器械开发的必要阶段。在小型动物模型中，兔子的血管口径相对较大，可用于血管移植物的吻合植入，作为进行大型动物研究之前的临床前测试。兔子与人具有相似的止血机制，可以适应不同部位不同直径的血管移植物，因此提供了评估小直径血管移植物的有效模型。本章将描述在兔颈动脉模型中进行生存手术的程序和所需材料，以进行小直径管状移植物的端侧和端端吻合技术的植入。
