Xia Yuan, Rao Zilong, Wu Simin, Huang Jiayao, Zhou Haiyun, Li Hanzhao, Zheng Hui, Guo Daxin, Quan Daping, Ou Jing-Song, Bai Ying, Liu Yunqi
Division of Cardiac Surgery, Cardiovascular Diseases Institute, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Guangdong Engineering Technology Research Centre for Functional Biomaterials, Key Laboratory for Polymeric Composite & Functional Materials of Ministry of Education, School of Materials Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China.
Regen Biomater. 2024 Aug 22;11:rbae098. doi: 10.1093/rb/rbae098. eCollection 2024.
Coronary artery bypass grafting is acknowledged as a major clinical approach for treatment of severe coronary artery atherosclerotic heart disease. This procedure typically requires autologous small-diameter vascular grafts. However, the limited availability of the donor vessels and associated trauma during tissue harvest underscore the necessity for artificial arterial alternatives. Herein, decellularized bovine intercostal arteries were successfully fabricated with lengths ranging from 15 to 30 cm, which also closely match the inner diameters of human coronary arteries. These decellularized arterial grafts exhibited great promise following poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) grafting from the inner surface. Such surface modification endowed the decellularized arteries with superior mechanical strength, enhanced anticoagulant properties and improved biocompatibility, compared to the decellularized bovine intercostal arteries alone, or even those decellularized grafts modified with both heparin and vascular endothelial growth factor. After replacement of the carotid arteries in rabbits, all surface-modified vascular grafts have shown good patency within 30 days post-implantation. Notably, strong signal was observed after α-SMA immunofluorescence staining on the PMPC-grafted vessels, indicating significant potential for regenerating the vascular smooth muscle layer and thereby restoring full structures of the artery. Consequently, the decellularized bovine intercostal arteries surface modified by PMPC can emerge as a potent candidate for small-diameter artificial blood vessels, and have shown great promise to serve as viable substitutes of arterial autografts.
冠状动脉旁路移植术被公认为是治疗严重冠状动脉粥样硬化性心脏病的主要临床方法。该手术通常需要自体小直径血管移植物。然而，供体血管的可用性有限以及组织采集过程中相关的创伤凸显了人工动脉替代品的必要性。在此，成功制备了长度为15至30厘米的去细胞牛肋间动脉，其内径也与人类冠状动脉内径密切匹配。这些去细胞动脉移植物在从内表面接枝聚（2-甲基丙烯酰氧基乙基磷酰胆碱）（PMPC）后展现出巨大潜力。与单独的去细胞牛肋间动脉相比，甚至与用肝素和血管内皮生长因子修饰的去细胞移植物相比，这种表面修饰赋予了去细胞动脉更高的机械强度、增强的抗凝性能和改善的生物相容性。在替换兔的颈动脉后，所有表面修饰的血管移植物在植入后30天内均显示出良好的通畅性。值得注意的是，在PMPC接枝血管上进行α-SMA免疫荧光染色后观察到强信号，表明在再生血管平滑肌层从而恢复动脉完整结构方面具有巨大潜力。因此，经PMPC表面修饰的去细胞牛肋间动脉可成为小直径人工血管的有力候选者，并已显示出作为动脉自体移植物可行替代品的巨大潜力。
