体外血脑屏障通透性的定量分析
Quantification of In Vitro Blood-Brain Barrier Permeability.
作者信息
Fu Bingmei M
机构信息
Department of Biomedical Engineering, The City College of the City University of New York, New York, NY, USA.
出版信息
Methods Mol Biol. 2022;2375:217-228. doi: 10.1007/978-1-0716-1708-3_18.
In vitro blood-brain barrier (BBB) models have been widely used to simulate in vivo models due to their low cost, feasibility, and repeatability. To serve as a valid substitute, the in vitro BBB should have the similar barrier function as that in vivo. This chapter summarizes the detailed methods for quantifying the barrier function, e.g., the permeability of the BBB to water, ions, and solutes for an in vitro BBB generated on the Transwell filter.
体外血脑屏障（BBB）模型因其成本低、可行性高和可重复性强而被广泛用于模拟体内模型。作为一种有效的替代物，体外血脑屏障应具有与体内相似的屏障功能。本章总结了量化屏障功能的详细方法，例如，对于在Transwell滤膜上生成的体外血脑屏障，测定其对水、离子和溶质的通透性。
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