Lindner J, Guenther J, Nick H, Zinser G, Antonicek H, Schachner M, Monard D
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4568-71. doi: 10.1073/pnas.83.12.4568.
Cultured explants from early postnatal mouse cerebellum were used to examine the influence of a 43-kDa glia-derived neurite-promoting factor (GdNPF) on the migration of [3H]thymidine-labeled granule cell neurons. GdNPF, which is a potent serine protease inhibitor, significantly reduced the extent of granule cell migration in a dose-dependent manner. This effect could be neutralized by addition of thrombin, which binds GdNPF. Other protease inhibitors such as aprotinin, hirudin, soybean trypsin inhibitor, leupeptin, 6-aminocaproic acid, and D-Phe-Pro-ArgCH2Cl do not show this inhibitory effect. These results demonstrate that a glia-derived protein can regulate the migration of postmitotic neurons, an important cellular event in the development of the nervous system.
利用出生后早期小鼠小脑的培养外植体来检测一种43 kDa的胶质细胞源性神经突促进因子（GdNPF）对[3H]胸腺嘧啶核苷标记的颗粒细胞神经元迁移的影响。GdNPF是一种有效的丝氨酸蛋白酶抑制剂，它以剂量依赖的方式显著降低了颗粒细胞的迁移程度。通过添加与GdNPF结合的凝血酶，可以中和这种作用。其他蛋白酶抑制剂，如抑肽酶、水蛭素、大豆胰蛋白酶抑制剂、亮抑酶肽、6-氨基己酸和D-苯丙氨酸-脯氨酸-精氨酸氯甲基酮，均未显示出这种抑制作用。这些结果表明，一种胶质细胞源性蛋白可以调节有丝分裂后神经元的迁移，这是神经系统发育中的一个重要细胞事件。
