Lubran M M
Ann Clin Lab Sci. 1978 Mar-Apr;8(2):106-10.
The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.
蛋白质的双缩脲反应为测量血清蛋白提供了一种简单而精确的方法；至少在每分升10克的范围内遵循比尔定律。有几种稳定的双缩脲试剂可供使用。血红蛋白是唯一重要的干扰因素，使用样品空白无法将其干扰降至最低。本文描述了双缩脲反应的机制，并提请注意血清蛋白的异质性以及使用经认证的白蛋白标准品。
