使用桑格法对临床样本中的新冠病毒全基因组进行测序。
Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method.
作者信息
Lu Roujian, Niu Peihua, Zhao Li, Wang Huijuan, Wang Wenling, Tan Wenjie
机构信息
Key Laboratory of Biosafety, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
出版信息
China CDC Wkly. 2020 Jun 19;2(25):447-452. doi: 10.46234/ccdcw2020.088.
Coronavirus disease 2019 (COVID-19), a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or COVID-19 virus, was reported in December 2019. Complete genomes of the COVID-19 virus from clinical samples using next generation sequencing (NGS) have been reported. Here we provide the technical data for sequencing complete genome of COVID-19 virus from clinical samples using the Sanger method. Two complete COVID-19 virus genome sequences (named WH19004-S and GX0002) were obtained from clinical samples of COVID-19 patients, and two single nucleotide polymorphisms (SNPs) in ORF7a (T/C, nt 27,493) and ORF8 (T/C, nt 28,253) of WH19004-S were identified by Sanger sequencing. The COVID-19 virus genome sequencing by Sanger method reported here could be used to generate data of high enough quality without requirement for expensive NGS equipment, which support sequencing complete genomes from clinical samples and monitoring of viral genetic variations of COVID-19 infections.
2019年冠状病毒病（COVID-19）是一种由名为严重急性呼吸综合征冠状病毒2（SARS-CoV-2）或COVID-19病毒的新型人类冠状病毒引起的疾病，于2019年12月被报道。已报道了使用下一代测序（NGS）从临床样本中获取的COVID-19病毒的完整基因组。在此，我们提供使用桑格法从临床样本中对COVID-19病毒完整基因组进行测序的技术数据。从COVID-19患者的临床样本中获得了两个完整的COVID-19病毒基因组序列（分别命名为WH19004-S和GX0002），并通过桑格测序法在WH19004-S的ORF7a（T/C，第27493位核苷酸）和ORF8（T/C，第28253位核苷酸）中鉴定出两个单核苷酸多态性（SNP）。本文报道的通过桑格法进行的COVID-19病毒基因组测序可用于生成足够高质量的数据，而无需昂贵的NGS设备，这有助于对临床样本进行完整基因组测序以及监测COVID-19感染的病毒基因变异。
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