Kisumi M, Nakanishi N, Takagi T, Chibata I
Appl Environ Microbiol. 1978 Feb;35(2):231-6. doi: 10.1128/aem.35.2.231-236.1978.
In Serratia marcescens, the mutation responsible for triazolealanine (TRA) resistance was transferred from a TRA-resistant mutant to a urocanase-less mutant by PS20-mediated transduction. The two crosses were performed using as donors two TRA-resistant mutants, whose phenotypes included increased levels of histidine-biosynthetic enzymes and feedback-insensitive phosphoribosyltransferase. In one cross, TRA-resistant transductants were urocanase-less mutants having only increased levels of the enzymes and barely detectable levels of urocanic acid. In the other cross, the transductants were urocanase-less mutants having both phenotypes of the donor, and most produced high concentrations (10.5 mg/ml) of urocanic acid.
在粘质沙雷氏菌中，通过PS20介导的转导作用，将负责三唑丙氨酸（TRA）抗性的突变从一个TRA抗性突变体转移到一个无尿刊酸酶的突变体中。使用两个TRA抗性突变体作为供体进行了两次杂交，这两个突变体的表型包括组氨酸生物合成酶水平升高以及对磷酸核糖基转移酶反馈不敏感。在一次杂交中，TRA抗性转导子是无尿刊酸酶的突变体，其酶水平仅有所升高，尿刊酸水平几乎检测不到。在另一次杂交中，转导子是无尿刊酸酶的突变体，具有供体的两种表型，并且大多数产生高浓度（10.5毫克/毫升）的尿刊酸。
