粘质沙雷氏菌苏氨酸生产菌株的转导构建
Transductional construction of a threonine-producing strain of Serratia marcescens.
作者信息
Komatsubara S, Kisumi M, Chibata I
出版信息
Appl Environ Microbiol. 1979 Dec;38(6):1045-51. doi: 10.1128/aem.38.6.1045-1051.1979.
Abstract
A threonine-producing strain of Serratia marcescens Sr41 was constructed according to the following process. Thr- strain E-60 was derived from strain HNr59 having constitutive levels of threonine-sensitive aspartokinase and homoserine dehydrogenase. Thr+ transductant T-570 was constructed from strain E-60 and phage grown on strain HNr21 having feedback-resistant threonine-sensitive aspartokinase and homoserine dehydrogenase. This transductant lacked both feedback inhibition and repression for the two enzymes. Thr- strain N-11 was derived from strain AECr174 lacking feedback inhibition and repression of lysine-sensitive aspartokinase. Subsequently, the threonine region of strain T-570 was transduced into strain N-11. One of the THR+ transductants, strain T-693, produced markedly high levels of the two aspartokinases and homoserine dehydrogenase, which were insensitive to feedback inhibition. This strain produced about 25 mg of threonine per ml in the medium containing sucrose and urea.
摘要
粘质沙雷氏菌Sr41的苏氨酸生产菌株是按照以下过程构建的。苏氨酸缺陷型菌株E - 60源自菌株HNr59，该菌株具有组成型水平的苏氨酸敏感型天冬氨酸激酶和高丝氨酸脱氢酶。苏氨酸原养型转导子T - 570是由菌株E - 60和在菌株HNr21上生长的噬菌体构建而成，菌株HNr21具有反馈抗性的苏氨酸敏感型天冬氨酸激酶和高丝氨酸脱氢酶。该转导子对这两种酶既缺乏反馈抑制也缺乏反馈阻遏。苏氨酸缺陷型菌株N - 11源自缺乏赖氨酸敏感型天冬氨酸激酶反馈抑制和阻遏的菌株AECr174。随后，将菌株T - 570的苏氨酸区域转导到菌株N - 11中。其中一个苏氨酸原养型转导子，菌株T - 693，产生了明显高水平的两种天冬氨酸激酶和高丝氨酸脱氢酶，它们对反馈抑制不敏感。该菌株在含有蔗糖和尿素的培养基中每毫升产生约25毫克苏氨酸。
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