Rhea Katherine
EXCET, Inc., Springfield, VA, USA.
US Army Combat Capabilities Development Command, Chemical Biological Center (DEVCOM-CBC), Edgewood, MD, USA.
Methods Mol Biol. 2022;2371:355-372. doi: 10.1007/978-1-0716-1689-5_19.
Bio-Layer Interferometry (BLI) enables the detection and characterization of molecular interactions in real-time without the hassle and interference of labeling. The affinity constant (K) obtained in the BLI analysis is an excellent indicator of quality of biomolecules such as antibodies, aptamers, peptides, etc. This method was used to analyze a peptide macrocycle against the Abrax protein, but can be used for any peptide macrocycle/analyte system.
生物层干涉术（BLI）能够实时检测和表征分子相互作用，而无需标记带来的麻烦和干扰。在BLI分析中获得的亲和常数（K）是抗体、适体、肽等生物分子质量的出色指标。该方法用于分析一种针对Abrax蛋白的肽大环，但可用于任何肽大环/分析物系统。
