在补充性平板培养基上亚致死热损伤鼠伤寒沙门氏菌的复苏
Recovery of sublethally heat-injured Salmonella typhimurium on supplemented plating media.
作者信息
D'Aoust J Y
出版信息
Appl Environ Microbiol. 1978 Mar;35(3):483-6. doi: 10.1128/aem.35.3.483-486.1978.
Abstract
The efficacy of 32 additives to Levine eosin-methylene blue-salts agar medium (EMBS) for the recovery of sublethally heat-injured Salmonella typhimurium was evaluated. In order of decreasing effectiveness, lactate, mannitol, and alpha-glycerophosphate mediated 90% or more recovery of injured cells; similar levels of recovery were obtained on EMBS supplemented with 1% (wt/vol) tryptic soy broth, protease peptone, or plate count agar. Other additives showed little or no capacity for repair or strongly inhibited heated and nonheated cell suspensions. Conditions of growth and storage before heat treatment were also found to markedly affect susceptibility to heat injury.
摘要
评估了32种添加剂对用于复苏亚致死热损伤鼠伤寒沙门氏菌的莱文伊红亚甲蓝盐琼脂培养基（EMBS）的效果。按有效性递减顺序，乳酸、甘露醇和α-甘油磷酸介导90%或更多损伤细胞的复苏；在添加1%（重量/体积）胰蛋白胨大豆肉汤、蛋白酶胨或平板计数琼脂的EMBS上获得了相似的复苏水平。其他添加剂对修复几乎没有或没有能力，或强烈抑制加热和未加热的细胞悬液。还发现热处理前的生长和储存条件显著影响对热损伤的敏感性。
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