Fodstad O, Brøgger A, Bruland O, Solheim O P, Nesland J M, Pihl A
Int J Cancer. 1986 Jul 15;38(1):33-40. doi: 10.1002/ijc.2910380107.
An osteosarcoma cell line, OHS, was established from a patient with multiple skeletal manifestations of osteosarcoma, developing after bilateral retinoblastoma. The tumor cells expressed sarcoma-associated antigens and showed rapid growth in monolayers and as multicellular spheroids. They formed distinct colonies in soft agar, and subcutaneous tumors in nude mice. Morphological studies indicated that OHS cells had retained important characteristics of the cells of origin. No deletion of the retinoblastoma genes on chromosome 13q14 could be demonstrated with the banding techniques used. However, cytogenetic studies revealed double minute chromosomes, as evidence of gene amplification, as well as translocations involving chromosomes 1,6,11 and 13. The OHS line can be used to study the genetic basis of tumor initiation and growth, and to elucidate factors predisposing for second primary cancers in retinoblastoma patients.
一种骨肉瘤细胞系OHS，是从一名双侧视网膜母细胞瘤后发生的、有多处骨肉瘤骨骼表现的患者身上建立的。肿瘤细胞表达肉瘤相关抗原，在单层培养和形成多细胞球体时生长迅速。它们在软琼脂中形成明显的集落，并在裸鼠体内形成皮下肿瘤。形态学研究表明，OHS细胞保留了起源细胞的重要特征。使用所采用的显带技术未发现13q14染色体上视网膜母细胞瘤基因的缺失。然而，细胞遗传学研究揭示了双微体染色体，作为基因扩增的证据，以及涉及1、6、11和13号染色体的易位。OHS细胞系可用于研究肿瘤发生和生长的遗传基础，并阐明视网膜母细胞瘤患者发生第二原发性癌症的易感因素。
