原癌基因在小鼠骨肉瘤中的表达。

Expression of protooncogenes in murine osteosarcomas.

作者信息

Schön A, Michiels L, Janowski M, Merregaert J, Erfle V

出版信息

Int J Cancer. 1986 Jul 15;38(1):67-74. doi: 10.1002/ijc.2910380112.

Abstract

The expression of 7 protooncogenes (c-sis, c-abl, c-mos, c-bas, c-Ki-ras, c-fos, c-myc) was examined in transplants and established cell lines from spontaneous and radiation-induced murine osteosarcomas. The transplant tumors were compared with different tissues, particularly skeletal tissue (sternum), and the osteosarcoma cell lines with fibroblast lines from the same mouse strains. C-sis was expressed above the level of controls in 2 osteosarcomas (TV, Os5). Three osteosarcomas showed over-expression of c-abl (TVK, DOS, Os5), c-bas (DOS, Os5 and V893) and c-fos (TVK, DOS, Os5), and 4 osteosarcomas showed over-expression of c-Ki-ras (TVK, DOS, Os5, Os16) and c-myc (TVK, DOS, TV, Os5). C-mos expression was not observed under the conditions used. One cell line (Os5) showed an altered transcript (1 kb transcript of c-fos). Apart from the relatively frequent increase in expression of the c-myc and c-ras-family, there was no indication that any particular protooncogene or combination of protooncogenes was associated with murine osteosarcomas.

摘要

在源自自发和辐射诱导的小鼠骨肉瘤的移植瘤及建立的细胞系中，检测了7种原癌基因（c-sis、c-abl、c-mos、c-bas、c-Ki-ras、c-fos、c-myc）的表达。将移植瘤与不同组织，特别是骨骼组织（胸骨）进行比较，并将骨肉瘤细胞系与来自相同小鼠品系的成纤维细胞系进行比较。在2例骨肉瘤（TV、Os5）中，c-sis的表达高于对照水平。3例骨肉瘤显示c-abl（TVK、DOS、Os5）、c-bas（DOS、Os5和V893）和c-fos（TVK、DOS、Os5）过表达，4例骨肉瘤显示c-Ki-ras（TVK、DOS、Os5、Os16）和c-myc（TVK、DOS、TV、Os5）过表达。在所采用的条件下未观察到c-mos表达。一个细胞系（Os5）显示转录本改变（c-fos的1 kb转录本）。除了c-myc和c-ras家族表达相对频繁增加外，没有迹象表明任何特定的原癌基因或原癌基因组合与小鼠骨肉瘤有关。