Sharpe E S, Bulla L A
Appl Environ Microbiol. 1978 Mar;35(3):601-9. doi: 10.1128/aem.35.3.601-609.1978.
Continuous culture of Bacillus popilliae was achieved for the first time in a small chemostat. Initially, variable cell yields during steady-state chemostat growth led to a re-examination of growth rates in batch cultures. B. popilliae NRRL B-2309 and a wild strain were both found to be natural mixtures of three substrains characterized by different growth rates and colony morphologies and varying stability. Selected subcultures grown continuously provided data for three different cell production curves. Cell yields were two to three times greater per unit of medium in continuous than in batch culture, and about 1% of slow-growing chemostat cells formed typical spores.
首次在小型恒化器中实现了日本金龟子芽孢杆菌的连续培养。最初，在恒化器稳态生长期间细胞产量的变化促使人们重新审视分批培养中的生长速率。结果发现，日本金龟子芽孢杆菌NRRL B - 2309和一株野生菌株均为三种亚菌株的天然混合物，这些亚菌株具有不同的生长速率、菌落形态和不同的稳定性。连续培养的选定亚培养物提供了三种不同细胞生产曲线的数据。连续培养中每单位培养基的细胞产量比分批培养高出两到三倍，并且约1%生长缓慢的恒化器细胞形成了典型的孢子。
