Nickerson K W, Bulla L A
Appl Microbiol. 1974 Jul;28(1):124-8. doi: 10.1128/am.28.1.124-128.1974.
A defined medium is described in which 18 strains of Bacillus thuringiensis representing the 12 established serotypes grow, sporulate, and produce a parasporal crystal. This minimal medium contains glucose and salts supplemented with either aspartate, glutamate, or citrate. These organic acids are required and cannot be replaced by vitamin mixtures or succinate even though succinate is taken up at a rate similar to that of aspartate, glutamate, and citrate.
本文描述了一种特定培养基,18株代表12个已确定血清型的苏云金芽孢杆菌菌株在此培养基中生长、形成芽孢并产生伴孢晶体。这种基本培养基含有葡萄糖和盐,并添加了天冬氨酸、谷氨酸或柠檬酸盐。这些有机酸是必需的,即使琥珀酸盐的吸收速率与天冬氨酸、谷氨酸和柠檬酸盐相似,也不能用维生素混合物或琥珀酸盐替代。