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本文引用的文献

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Revising CX3CR1 Expression on Murine Classical and Non-classical Monocytes.修订小鼠经典和非经典单核细胞上的 CX3CR1 表达。
Front Immunol. 2020 Jun 3;11:1117. doi: 10.3389/fimmu.2020.01117. eCollection 2020.
2
Effect of Nivolumab vs Bevacizumab in Patients With Recurrent Glioblastoma: The CheckMate 143 Phase 3 Randomized Clinical Trial.纳武利尤单抗对比贝伐珠单抗治疗复发性胶质母细胞瘤患者的效果：CheckMate 143 期随机临床试验。
JAMA Oncol. 2020 Jul 1;6(7):1003-1010. doi: 10.1001/jamaoncol.2020.1024.
3
Dissociation of solid tumor tissues with cold active protease for single-cell RNA-seq minimizes conserved collagenase-associated stress responses.使用冷激活蛋白酶分离固体肿瘤组织进行单细胞 RNA-seq 最小化了保守的胶原酶相关应激反应。
Genome Biol. 2019 Oct 17;20(1):210. doi: 10.1186/s13059-019-1830-0.
4
OMIP-061: 20-Color Flow Cytometry Panel for High-Dimensional Characterization of Murine Antigen-Presenting Cells.OMIP-061：用于检测小鼠抗原呈递细胞的高维特征的 20 色流式细胞术面板。
Cytometry A. 2019 Dec;95(12):1226-1230. doi: 10.1002/cyto.a.23880. Epub 2019 Aug 19.
5
Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma.新辅助抗 PD-1 免疫治疗在复发性胶质母细胞瘤中促进了肿瘤内和全身免疫应答，并带来生存获益。
Nat Med. 2019 Mar;25(3):477-486. doi: 10.1038/s41591-018-0337-7. Epub 2019 Feb 11.
6
OMIP-054: Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass Cytometry.OMIP-054：通过质谱细胞术对原位小鼠脑胶质瘤模型的大脑、脾脏和骨髓中的固有和适应性白细胞进行广谱免疫表型分析。
Cytometry A. 2019 Apr;95(4):422-426. doi: 10.1002/cyto.a.23725. Epub 2019 Jan 31.
7
Effect of Tumor-Treating Fields Plus Maintenance Temozolomide vs Maintenance Temozolomide Alone on Survival in Patients With Glioblastoma: A Randomized Clinical Trial.肿瘤治疗电场联合维持性替莫唑胺与单纯维持性替莫唑胺对胶质母细胞瘤患者生存的影响：一项随机临床试验
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8
The Microenvironmental Landscape of Brain Tumors.脑肿瘤的微环境格局
Cancer Cell. 2017 Mar 13;31(3):326-341. doi: 10.1016/j.ccell.2017.02.009.
9
OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland.OMIP - 032：用于评估小鼠乳腺中固有免疫细胞和适应性免疫细胞的两种多色免疫表型分析面板。
Cytometry A. 2016 Jun;89(6):527-30. doi: 10.1002/cyto.a.22867. Epub 2016 May 23.
10
A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflamed Murine Non-Lymphoid Tissues.正常和炎症小鼠非淋巴组织中免疫细胞综合流式细胞术分析方案
PLoS One. 2016 Mar 3;11(3):e0150606. doi: 10.1371/journal.pone.0150606. eCollection 2016.

对鼠脑肿瘤微环境进行广泛的免疫表型分析。

Broad immunophenotyping of the murine brain tumor microenvironment.

机构信息

Duke School of Medicine, Department of Immunology, United States of America.

Duke School of Medicine, Department of Pathology, United States of America.

出版信息

J Immunol Methods. 2021 Dec;499:113158. doi: 10.1016/j.jim.2021.113158. Epub 2021 Sep 29.

DOI:10.1016/j.jim.2021.113158
PMID:34597618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8608727/
Abstract

Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.

摘要

在这里，我们展示了一个用于评估小鼠脑胶质瘤样本中 13 种髓系和淋巴系群体的 14 色流式细胞术面板。试剂、处理方案和下游分析经过了彻底的验证和优化，以解决以下群体：T 细胞（CD4、CD8、CD3）、B 细胞（B220）、NK 细胞（NK1.1）、中性粒细胞（Ly6G）、经典和非经典单核细胞（Ly6c、CD43）、巨噬细胞（F4/80、CD11b）、小胶质细胞（CD45-lo、CD11b）和树突状细胞（DCs）（CD11c、MHC Ⅱ类）。此外，该面板为荧光报告基因或同基因标记染色留出了 Alexa Fluor 488/FITC 的空位。