对鼠脑肿瘤微环境进行广泛的免疫表型分析。
Broad immunophenotyping of the murine brain tumor microenvironment.
机构信息
Duke School of Medicine, Department of Immunology, United States of America.
Duke School of Medicine, Department of Pathology, United States of America.
出版信息
J Immunol Methods. 2021 Dec;499:113158. doi: 10.1016/j.jim.2021.113158. Epub 2021 Sep 29.
Abstract
Here we present a 14-color flow cytometry panel for the evaluation of 13 myeloid and lymphoid populations within murine glioblastoma samples. Reagents, processing protocols, and downstream analyses were thoroughly validated and optimized to resolve the following populations: T cells (CD4, CD8, CD3), B cells (B220), NK cells (NK1.1), neutrophils (Ly6G), classical and non-classical monocytes (Ly6c, CD43), macrophages (F4/80, CD11b), microglia (CD45-lo, CD11b), and dendritic cells (DCs) (CD11c, MHC class II). In addition, this panel leaves Alexa Fluor 488/FITC open for the inclusion of fluorescent reporters or congenic marker staining.
摘要
在这里，我们展示了一个用于评估小鼠脑胶质瘤样本中 13 种髓系和淋巴系群体的 14 色流式细胞术面板。试剂、处理方案和下游分析经过了彻底的验证和优化，以解决以下群体：T 细胞（CD4、CD8、CD3）、B 细胞（B220）、NK 细胞（NK1.1）、中性粒细胞（Ly6G）、经典和非经典单核细胞（Ly6c、CD43）、巨噬细胞（F4/80、CD11b）、小胶质细胞（CD45-lo、CD11b）和树突状细胞（DCs）（CD11c、MHC Ⅱ类）。此外，该面板为荧光报告基因或同基因标记染色留出了 Alexa Fluor 488/FITC 的空位。
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