Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, Guangxi, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China.
Plant Dis. 2022 Feb;106(2):741-744. doi: 10.1094/PDIS-07-21-1565-A. Epub 2022 Feb 7.
pv. is the causal agent of bacterial blight, one of the most devastating diseases of rice. Here, a hypervirulent strain, C9-3, defeating , , , and resistance genes, was used to extract genomic DNA for single molecule real-time (SMRT) sequencing. After assembly, the genome consists of a single-circular chromosome with the size of 4,924,298 bp with G+C content of 63.7% and contains 4,715 genes. Annotation and analysis of the genes using a suite of applications named AnnoTALE suggested that 17 transcription activator-like effectors, including 15 typical TALEs and 2 iTALEs/truncTALEs, were encoded in the genome. The approach and genome resource will contribute to the discovery of new virulence effectors and understanding on rice-. pv. interactions.
pv. 是细菌性枯萎病的病原体，这是水稻最具破坏性的疾病之一。在这里，使用了一种高毒力菌株 C9-3 来提取基因组 DNA 进行单分子实时 (SMRT) 测序。组装后，基因组由一个大小为 4924298bp 的单环染色体组成，G+C 含量为 63.7%，包含 4715 个基因。使用名为 AnnoTALE 的一套应用程序对 基因进行注释和分析表明，该基因组编码了 17 个转录激活样效应子，包括 15 个典型的 TALEs 和 2 个 iTALEs/truncTALEs。该方法和基因组资源将有助于发现新的毒力效应子，并深入了解水稻与. pv. 的相互作用。
