Frohn A, Födisch H J, Bode U
Klin Padiatr. 1986 May-Jun;198(3):262-6. doi: 10.1055/s-2008-1026887.
The single-cell fluorescence cytometric data presented in this study basing on measurements on 6 osseous Ewing's-Sarcoma and 17 classical osteosarcoma are showing a mirrorlike behaviour to cytological and histological appearance. The cellular monotony in Ewing's-Sarcoma and the polymorphic appearance of osteosarcoma are represented by typical aneuploid DNA-histograms. Ewing's-Sarcoma histogram's show a large peak between the diploid and tetraploid region and little deviation to the octoploid value. The conventional osteosarcomas are characterised by a very polyploid and changeable DNA-histogram with variable blocks and peaks and deviation up to 34c. Special trends in the subtypes are remarkable, but depend on further investigations. DNA-measurements support objectivization of histological appearance on the one hand and the controlling of therapeutical responses on the other hand.
本研究基于对6例骨尤文肉瘤和17例经典骨肉瘤的测量得出的单细胞荧光细胞计量数据，显示出与细胞学和组织学外观呈镜像关系。尤文肉瘤中的细胞单一性和骨肉瘤的多形性外观由典型的非整倍体DNA直方图表示。尤文肉瘤直方图在二倍体和四倍体区域之间显示出一个大峰，与八倍体值的偏差很小。传统骨肉瘤的特征是具有非常多倍体且可变的DNA直方图，有可变的块和峰，偏差高达34c。亚型中的特殊趋势很显著，但取决于进一步的研究。DNA测量一方面支持组织学外观的客观化，另一方面支持对治疗反应的控制。
