Department of Otolaryngology-Head and Neck Surgery, Upstate Medical University, Syracuse, New York, U.S.A.
College of Medicine, Upstate Medical University, Syracuse, New York, U.S.A.
Laryngoscope. 2022 Mar;132(3):648-654. doi: 10.1002/lary.29887. Epub 2021 Oct 2.
To evaluate histologic changes in middle ear and eustachian tube (ET) mucosa of mice after exposure to tobacco or electronic cigarette (e-cigarette) smoke. To determine whether there were any mitigating effects of middle ear application of anti-IL-13 or the epidermal growth factor receptor antagonist AG1478 on noted changes within ET mucosa.
Controlled animal study.
Fifty BALB/cJ mice were randomly assigned to one of five groups: A control group with no smoke exposure, two groups exposed to tobacco smoke, and two groups exposed to e-cigarette vapor. Within the exposed groups after 4 weeks of exposure, one ear was infiltrated with a saline hydrogel and the other ear with hydrogel of either Anti-IL-13 or AG1478. After four more weeks of exposure, the animals were euthanized and the ETs were evaluated for mucosal changes.
Compared to control animals with no smoke exposure, there were significant decreases in the numbers of goblet cells within the ET mucosa of mice exposed to tobacco smoke and e-cigarette vapor. No significant differences in cilia, mucin, or squamous metaplasia were noted. Neither anti-IL-13 nor AG178 significantly altered goblet cell count in the ET mucosa of mice exposed to tobacco smoke; however, both agents significantly increased goblet cells within the ET mucosa of mice exposed to e-cigarette vapor.
Short-term tobacco smoke and e-cigarette vapor significantly decrease goblet cell count in mouse ET mucosa. Middle ear application of both anti-IL-13 and AG1478 resulted in an increase in goblet cell count among mice exposed to e-cigarette vapor, but not to tobacco smoke.
NA Laryngoscope, 132:648-654, 2022.
评估暴露于烟草或电子烟烟雾后中耳和咽鼓管（ET）黏膜的组织学变化。确定中耳应用抗白细胞介素 13（IL-13）或表皮生长因子受体拮抗剂 AG1478 是否对 ET 黏膜内观察到的变化有任何缓解作用。
对照动物研究。
将 50 只 BALB/cJ 小鼠随机分为五组之一：无烟雾暴露的对照组、两组暴露于烟草烟雾、两组暴露于电子烟蒸气。在暴露组中，经过 4 周的暴露后，一只耳朵用盐水水凝胶浸润，另一只耳朵用抗 IL-13 或 AG1478 的水凝胶浸润。在进一步暴露四周后，处死动物并评估 ET 黏膜的变化。
与未暴露于烟雾的对照动物相比，暴露于烟草烟雾和电子烟蒸气的小鼠 ET 黏膜中的杯状细胞数量明显减少。纤毛、粘蛋白或鳞状化生无明显差异。抗 IL-13 或 AG178 均未显著改变暴露于烟草烟雾的小鼠 ET 黏膜中的杯状细胞计数；然而，两种药物都显著增加了暴露于电子烟蒸气的小鼠 ET 黏膜中的杯状细胞数量。
短期烟草烟雾和电子烟蒸气显著减少了小鼠 ET 黏膜中的杯状细胞数量。中耳应用抗 IL-13 和 AG1478 均可增加暴露于电子烟蒸气的小鼠 ET 黏膜中的杯状细胞数量，但对烟草烟雾无影响。
无 喉镜，132:648-654,2022.
