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Use of oligonucleotides to generate large deletions.

作者信息

Eghtedarzadeh M K, Henikoff S

出版信息

Nucleic Acids Res. 1986 Jun 25;14(12):5115. doi: 10.1093/nar/14.12.5115.

DOI:10.1093/nar/14.12.5115
PMID:3460029
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311518/
Abstract
摘要

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1
Use of oligonucleotides to generate large deletions.使用寡核苷酸产生大片段缺失。
Nucleic Acids Res. 1986 Jun 25;14(12):5115. doi: 10.1093/nar/14.12.5115.
2
Use of lambda exonuclease for efficient oligonucleotide-mediated site-directed deletion and point mutation of double-stranded DNA.使用λ核酸外切酶进行高效的寡核苷酸介导的双链DNA定点缺失和点突变。
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3
[Application of synthetic DNA fragments in molecular biology].
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4
Using DNAWorks in designing oligonucleotides for PCR-based gene synthesis.利用DNAWorks设计用于基于PCR的基因合成的寡核苷酸。
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5
[Mutagenesis directed by phosphotriester analogs of oligonucleotides].
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The gapped duplex DNA approach to oligonucleotide-directed mutation construction.用于寡核苷酸定向突变构建的缺口双链DNA方法。
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In vitro generation of specific deletions in DNA cloned in M13 vectors using synthetic oligodeoxyribonucleotides: mutants in the 5'-flanking region of the yeast alcohol dehydrogenase II gene.利用合成寡脱氧核糖核苷酸在克隆于M13载体的DNA中体外产生特定缺失：酵母乙醇脱氢酶II基因5'侧翼区的突变体
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Increased efficiency of oligonucleotide mutagenesis by removal of endogenous primers from preparations of single-stranded M13 DNA.
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本文引用的文献

1
pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
2
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Methods Enzymol. 1983;100:333-42. doi: 10.1016/0076-6879(83)00066-x.