School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Feb 5;266:120407. doi: 10.1016/j.saa.2021.120407. Epub 2021 Sep 20.
Hydrogen sulfide (HS), a recognized environmental pollutant, comes from a wide range of sources. For example, HS will be produced in the process of plant protein corruption, the decomposition of domestic sewage and garbage, food processing (wine brewing), etc. and once the concentration is too high, it will cause significant damage of environment and human body. Besides HS is an important gas signal molecule in vivo, which can be transferred through lipid membrane. Its existence level is closely related to many diseases. If we can "visually" trace the transmembrane transmission of hydrogen sulfide, it will be very helpful for the study of oxidative stress processes, cell protection, signal transduction and related diseases closely related to HS. Although some probes can detect HS in environment, cytoplasm and organelles, there are few reports on the release and internalization of HS. In this work, we report a HS fluorescence probe that can retain on the cell membrane, named PCM. The probe PCM can not only detect endogenous and exogenous HS, but also distinguish them, this provides a general strategy for the construction of probes to detect other biomarkers. In addition, PCM has been successfully applied to the detection of endogenous and exogenous HS in zebrafish, which has the potential to become a new chemical tool and provide help for the research of HS-related diseases.
硫化氢（HS）是一种公认的环境污染物，来源于广泛的来源。例如，HS 会在植物蛋白腐败、生活污水和垃圾分解、食品加工（酿酒）等过程中产生，一旦浓度过高，就会对环境和人体造成严重损害。此外，HS 是体内一种重要的气体信号分子，可以通过脂质膜传递。它的存在水平与许多疾病密切相关。如果我们能够“直观”地追踪硫化氢的跨膜传递，这将对氧化应激过程、细胞保护、信号转导以及与 HS 密切相关的相关疾病的研究非常有帮助。尽管有些探针可以检测环境、细胞质和细胞器中的 HS，但关于 HS 的释放和内化的报道很少。在这项工作中，我们报告了一种可以保留在细胞膜上的 HS 荧光探针，命名为 PCM。探针 PCM 不仅可以检测内源性和外源性 HS，还可以区分它们，这为构建用于检测其他生物标志物的探针提供了一种通用策略。此外，PCM 已成功应用于斑马鱼内源性和外源性 HS 的检测，它有可能成为一种新的化学工具，并为 HS 相关疾病的研究提供帮助。
