State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, China.
State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, China.
Talanta. 2024 Jan 1;266(Pt 1):125043. doi: 10.1016/j.talanta.2023.125043. Epub 2023 Aug 5.
Hydrogen sulfide (HS) acts as a gas transporter and cell protector and plays a role in a number of disorders and signaling processes. Given that the half-life of HS in biological systems is between seconds and minutes, the development of rapid and accurate technologies for reliable monitoring HS levels and dynamics in organisms is critical. However, it is still difficult to design innovative near-infrared fluorescent probes that can quickly and accurately detect HS. Here, we constructed a novel NIR ratiometric fluorescent probe based on the "aldehyde group auxiliary strategy", Cy-HS, for the quantitative detection and precise imaging of HS in living cells and zebrafish. Cy-HS responded quickly (150 s) and was highly sensitive (0.179 μM) to HS donor. Cy-HS was further successfully employed to track endogenous HS fluctuation in HCT116 cells and zebrafish and evaluated the release efficiency of the HS prodrug in a NIR ratiometric imaging way. Cy-HS has the potential to be used as a reliable indication of HS levels in living cells and zebrafish, as well as an innovative and practical instrument for furthering the physiological research of HS, which will encourage the creation of advanced NIR ratiometric probes for a variety of biological applications.
硫化氢 (HS) 作为一种气体转运体和细胞保护剂，在多种疾病和信号转导过程中发挥作用。鉴于 HS 在生物体系中的半衰期在秒到分钟之间，因此开发快速、准确的技术以可靠监测生物体中 HS 水平和动态变化至关重要。然而，设计能够快速、准确检测 HS 的创新型近红外荧光探针仍然具有挑战性。在这里，我们构建了一种新型的基于“醛基辅助策略”的近红外比率型荧光探针 Cy-HS，用于活细胞和斑马鱼中 HS 的定量检测和精确成像。Cy-HS 对 HS 供体的响应速度快（150 s），灵敏度高（0.179 μM）。Cy-HS 进一步成功地用于跟踪 HCT116 细胞和斑马鱼内源性 HS 的波动，并以近红外比率成像的方式评估 HS 前药的释放效率。Cy-HS 有望成为活细胞和斑马鱼中 HS 水平的可靠指示剂，以及用于进一步研究 HS 生理学的创新实用工具，这将鼓励开发用于各种生物应用的先进近红外比率型探针。
