State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China.
J Hazard Mater. 2022 Feb 15;424(Pt A):127223. doi: 10.1016/j.jhazmat.2021.127223. Epub 2021 Sep 21.
Pyrisoxazole, an isoxazoline-class fungicide, has been registered and used for approximately 19 years. However, its environmental transformation products (TPs) and corresponding ecotoxicological effects remain ambiguous. In this study, the photolysis, hydrolysis, and soil transformation behavior of pyrisoxazole were systematically investigated by indoor simulation experiments and analyzed by liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS) and UNIFI software. Transformation products in different environemnts were effectively identfied by a proposed workflow, which organically combined suspect and non-target screening strategies. In total, 17 TPs were screened out. Eight TPs were confirmed using the corresponding reference standards. Structures of another 9 compounds were tentatively proposed based on diagnostic evidence. Among them, 14 products were reported for the first time. The transformation pathways of pyrisoxazole in soil and water were proposed. Pathway analysis demonstrated that the different pH of aqueous solutions had little effect on the pathways, while the influence of different soil types and oxygen conditions was evident. Finally, the toxicity of the proposed TPs to fish and daphnids was predicted using ECOSAR software. These proposed TPs in soil and water, transformation pathways, and predicted ecotoxicity information could provide systematic insight into the fate and environmental risks of pyrisoxazole.
吡唑醚菌酯，一种异噁唑啉类杀菌剂，已注册并使用了大约 19 年。然而，其环境转化产物（TPs）及其相应的生态毒理学效应仍不清楚。本研究通过室内模拟实验系统研究了吡唑醚菌酯的光解、水解和土壤转化行为，并通过液相色谱四极杆飞行时间质谱（LC-QTOF-MS）和 UNIFI 软件进行了分析。通过提出的工作流程，有效地鉴定了不同环境中的转化产物，该工作流程有机地结合了嫌疑和非靶向筛选策略。总共筛选出 17 种 TPs。使用相应的标准品确认了其中 8 种 TPs。根据诊断证据，对另外 9 种化合物的结构进行了初步推测。其中，有 14 种产品是首次报道的。提出了吡唑醚菌酯在土壤和水中的转化途径。途径分析表明，水溶液的不同 pH 值对途径几乎没有影响，而不同土壤类型和氧气条件的影响则很明显。最后，使用 ECOSAR 软件预测了这些提出的 TPs 对鱼类和水蚤的毒性。这些在土壤和水中的提出的 TPs、转化途径和预测的生态毒性信息可以为吡唑醚菌酯的命运和环境风险提供系统的了解。
