外源性谷胱甘肽可保护肠道上皮细胞免受氧化损伤。
Exogenous glutathione protects intestinal epithelial cells from oxidative injury.
作者信息
Lash L H, Hagen T M, Jones D P
出版信息
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4641-5. doi: 10.1073/pnas.83.13.4641.
Abstract
Exogenous GSH provided rat small-intestinal epithelial cells with significant protection against injury induced by t-butyl hydroperoxide or menadione. This protection was found to be dependent upon uptake of intact GSH. Uptake of GSH occurred by a Na+-dependent electrogenic system found in the basolateral membrane. Thus, rat small-intestinal epithelial cells can utilize plasma GSH to support intracellular detoxication systems that function in protection against chemically induced injury.
摘要
外源性谷胱甘肽（GSH）为大鼠小肠上皮细胞提供了显著的保护作用，使其免受叔丁基过氧化氢或甲萘醌诱导的损伤。这种保护作用依赖于完整GSH的摄取。GSH的摄取是通过位于基底外侧膜上的一种依赖钠离子的电驱动系统进行的。因此，大鼠小肠上皮细胞可以利用血浆中的GSH来支持细胞内的解毒系统，该系统在抵御化学诱导损伤中发挥作用。
相似文献
1
Exogenous glutathione protects intestinal epithelial cells from oxidative injury.外源性谷胱甘肽可保护肠道上皮细胞免受氧化损伤。
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4641-5. doi: 10.1073/pnas.83.13.4641.
2
Glutathione uptake and protection against oxidative injury in isolated kidney cells.离体肾细胞中谷胱甘肽的摄取及对氧化损伤的保护作用
Kidney Int. 1988 Jul;34(1):74-81. doi: 10.1038/ki.1988.147.
3
Oxidative stress in isolated rat renal proximal and distal tubular cells.分离的大鼠肾近端和远端肾小管细胞中的氧化应激
Am J Physiol. 1990 Aug;259(2 Pt 2):F338-47. doi: 10.1152/ajprenal.1990.259.2.F338.
4
Protection of retinal pigment epithelium from oxidative injury by glutathione and precursors.谷胱甘肽及其前体对视网膜色素上皮细胞氧化损伤的保护作用。
Invest Ophthalmol Vis Sci. 1993 Dec;34(13):3661-8.
5
Protection from oxidant injury by sodium-dependent GSH uptake in retinal Müller cells.视网膜Müller细胞中钠依赖性谷胱甘肽摄取对氧化损伤的保护作用。
Exp Eye Res. 1999 May;68(5):609-16. doi: 10.1006/exer.1998.0639.
6
Protection of cultured gastric cells against tert-butyl hydroperoxide by glutathione isopropyl ester.谷胱甘肽异丙酯对培养的胃细胞免受叔丁基过氧化氢的损伤作用。
Eur J Pharmacol. 1998 Jun 26;351(3):363-9. doi: 10.1016/s0014-2999(98)00320-3.
7
Cytotoxicity of an organic hydroperoxide and cellular antioxidant defense system against hydroperoxides in cultured mammalian cells.一种有机氢过氧化物的细胞毒性以及培养的哺乳动物细胞中针对氢过氧化物的细胞抗氧化防御系统
Toxicology. 1989 Apr;55(1-2):69-82. doi: 10.1016/0300-483x(89)90175-3.
8
Protection of rat hepatocytes from tert-butyl hydroperoxide-induced injury by catechol.邻苯二酚对叔丁基过氧化氢诱导的大鼠肝细胞损伤的保护作用
Toxicol Appl Pharmacol. 1986 Jul;84(3):607-16. doi: 10.1016/0041-008x(86)90267-x.
9
Deterioration of membrane morphology, phospholipids, and cytoskeletal protein in rat erythrocytes exposed to tert-butyl hydroperoxide: protection by exogenous glutathione fails in selenium deficiency.叔丁基过氧化氢作用下大鼠红细胞的膜形态、磷脂和细胞骨架蛋白的恶化：在硒缺乏时外源性谷胱甘肽的保护作用失效
J Nutr Sci Vitaminol (Tokyo). 1988 Oct;34(5):491-506. doi: 10.3177/jnsv.34.491.
10
Hepatoprotective activity of xanthones and xanthonolignoids against tert-butylhydroperoxide-induced toxicity in isolated rat hepatocytes--comparison with silybin.氧杂蒽酮和氧杂蒽酮木脂素对叔丁基过氧化氢诱导的离体大鼠肝细胞毒性的保肝活性——与水飞蓟宾的比较
Pharm Res. 1995 Nov;12(11):1756-60. doi: 10.1023/a:1016230125496.
引用本文的文献
1
Glutathione has cell protective and anti-catabolic effects in articular cartilage without impairing the chondroanabolic phenotype.谷胱甘肽在关节软骨中具有细胞保护和抗分解代谢作用，且不会损害软骨合成代谢表型。
Heliyon. 2024 Nov 13;10(22):e40368. doi: 10.1016/j.heliyon.2024.e40368. eCollection 2024 Nov 30.
2
Effects of Dietary Supplementation with Glutamine on the Immunity and Intestinal Barrier Gene Expression in Broiler Chickens Infected with Enteritidis.日粮添加谷氨酰胺对感染肠炎沙门氏菌肉鸡免疫力及肠道屏障基因表达的影响
Animals (Basel). 2022 Aug 24;12(17):2168. doi: 10.3390/ani12172168.
3
Polymerized Whey Protein Concentrate-Based Glutathione Delivery System: Physicochemical Characterization, Bioavailability and Sub-Chronic Toxicity Evaluation.基于聚合乳清蛋白浓缩物的谷胱甘肽递送系统：理化特性、生物利用度及亚慢性毒性评估
Molecules. 2021 Mar 24;26(7):1824. doi: 10.3390/molecules26071824.
4
Ultrasensitive magnetic resonance imaging of systemic reactive oxygen species for early diagnosis of sepsis using activatable nanoprobes.使用可激活纳米探针进行全身活性氧的超灵敏磁共振成像以早期诊断败血症
Chem Sci. 2019 Feb 21;10(13):3770-3778. doi: 10.1039/c8sc04961k. eCollection 2019 Apr 7.
5
Glucose-dependent anaplerosis in cancer cells is required for cellular redox balance in the absence of glutamine.在没有谷氨酰胺的情况下，癌细胞中葡萄糖依赖性氨酰生成对于细胞氧化还原平衡是必需的。
Sci Rep. 2016 Sep 8;6:32606. doi: 10.1038/srep32606.
6
Detection of oxidative stress-induced carbonylation in live mammalian cells.活的哺乳动物细胞中氧化应激诱导的羰基化检测。
Free Radic Biol Med. 2015 Jul;84:11-21. doi: 10.1016/j.freeradbiomed.2015.03.011. Epub 2015 Mar 20.
7
Glutathione-mediated detoxification of halobenzoquinone drinking water disinfection byproducts in T24 cells.谷胱甘肽介导的T24细胞中卤代苯醌类饮用水消毒副产物的解毒作用
Toxicol Sci. 2014 Oct;141(2):335-43. doi: 10.1093/toxsci/kfu088. Epub 2014 May 8.
8
Effect of partially protected butyrate used as feed additive on growth and intestinal metabolism in sea bream (Sparus aurata).部分保护丁酸盐作为饲料添加剂对真鲷生长和肠道代谢的影响。
Fish Physiol Biochem. 2013 Dec;39(6):1567-80. doi: 10.1007/s10695-013-9809-3. Epub 2013 Jun 5.
9
Current status and emerging role of glutathione in food grade lactic acid bacteria.谷胱甘肽在食品级乳酸菌中的现状及新兴作用。
Microb Cell Fact. 2012 Aug 25;11:114. doi: 10.1186/1475-2859-11-114.
10
Redox biology of the intestine.肠道的氧化还原生物学。
Free Radic Res. 2011 Nov;45(11-12):1245-66. doi: 10.3109/10715762.2011.611509. Epub 2011 Sep 5.
本文引用的文献
1
Physiological significance of glutathione S-transferases.谷胱甘肽S-转移酶的生理意义。
Am J Physiol. 1980 Dec;239(6):G439-44. doi: 10.1152/ajpgi.1980.239.6.G439.
2
High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides.谷胱甘肽、二硫化谷胱甘肽及相关硫醇和二硫化物纳摩尔水平的高效液相色谱分析。
Anal Biochem. 1980 Jul 15;106(1):55-62. doi: 10.1016/0003-2697(80)90118-9.
3
Metabolism of hydrogen peroxide in isolated hepatocytes: relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated H2O2.分离的肝细胞中过氧化氢的代谢：过氧化氢酶和谷胱甘肽过氧化物酶在内源性生成的H2O2分解中的相对作用。
Arch Biochem Biophys. 1981 Sep;210(2):505-16. doi: 10.1016/0003-9861(81)90215-0.
4
Protective role of the glutathione redox cycle against adriamycin-mediated toxicity in isolated hepatocytes.谷胱甘肽氧化还原循环对阿霉素介导的离体肝细胞毒性的保护作用。
Biochem Pharmacol. 1981 Aug 15;30(16):2299-304. doi: 10.1016/0006-2952(81)90102-7.
5
The mechanism of biliary secretion of reduced glutathione. Analysis of transport process in isolated rat-liver canalicular membrane vesicles.还原型谷胱甘肽的胆汁分泌机制。对分离的大鼠肝胆小管膜囊泡转运过程的分析。
Eur J Biochem. 1983 Aug 15;134(3):467-71. doi: 10.1111/j.1432-1033.1983.tb07590.x.
6
Transport of glutathione by renal basal-lateral membrane vesicles.谷胱甘肽经肾基底外侧膜囊泡的转运
Biochem Biophys Res Commun. 1983 Apr 15;112(1):55-60. doi: 10.1016/0006-291x(83)91796-5.
7
Regulation of intracellular calcium compartmentation: studies with isolated hepatocytes and t-butyl hydroperoxide.细胞内钙区隔化的调节：对分离的肝细胞和叔丁基过氧化氢的研究
Proc Natl Acad Sci U S A. 1982 Nov;79(22):6842-6. doi: 10.1073/pnas.79.22.6842.
8
Glutathione transport across hepatocyte plasma membranes. Analysis using isolated rat-liver sinusoidal-membrane vesicles.谷胱甘肽跨肝细胞质膜的转运。使用分离的大鼠肝脏窦状隙膜囊泡进行的分析。
Eur J Biochem. 1984 Feb 1;138(3):491-5. doi: 10.1111/j.1432-1033.1984.tb07943.x.
9
Characterization of the membrane-associated thiol oxidase activity of rat small-intestinal epithelium.大鼠小肠上皮细胞膜相关硫醇氧化酶活性的表征
Arch Biochem Biophys. 1983 Aug;225(1):344-52. doi: 10.1016/0003-9861(83)90039-5.
10
Transport of glutathione by intestinal brush border membrane vesicles.谷胱甘肽通过肠刷状缘膜囊泡的转运。
Biochem Biophys Res Commun. 1984 Sep 28;123(3):929-36. doi: 10.1016/s0006-291x(84)80223-5.
Zotero 插件