黑醋栗叶总黄酮、芦丁和异槲皮苷对离体兔心脏前列腺素生物合成及释放的比较作用。

Comparative effects of total flavonoids extracted from Ribes nigrum leaves, rutin and isoquercitrin on biosynthesis and release of prostaglandins in the ex vivo rabbit heart.

作者信息

Chanh P H, Ifansyah N, Chahine R, Mounayar-Chalfoun A, Gleye J, Moulis C

出版信息

Prostaglandins Leukot Med. 1986 Jun;22(3):295-300. doi: 10.1016/0262-1746(86)90140-x.

Abstract

Experiments were carried out on isolated rabbit hearts: PG biosynthesis was induced by administration into the coronary circulation of a single dose of arachidonic acid (100 micrograms) and the PGs synthesized and released in the heart effluent were detected by bioassays. Total flavonoids (TF) extracted from Ribes nigrum leaves and their 2 major components, rutin and isoquercitrin had neither spasmodic nor relaxing activity on rat stomach strip. They were not capable of inducing any biosynthesis and release of PG-like substances and did not act on PGE2 receptors. They inhibited both biosynthesis and release of PG-like substances: TF were more active than rutin and isoquercitrin: ID30 was 1.03 +/- 0.24 mg/ml for TF versus 3.75 +/- 0.24 mg/ml and 2.31 +/- 1.4 mg/ml for rutin and isoquercitrin respectively.

摘要

在离体兔心脏上进行了实验

通过向冠状动脉循环中单次注射花生四烯酸（100微克）诱导前列腺素（PG）生物合成，并通过生物测定法检测心脏流出液中合成和释放的PG。从黑醋栗叶中提取的总黄酮（TF）及其两种主要成分芦丁和异槲皮苷对大鼠胃条既无痉挛作用也无松弛作用。它们不能诱导任何PG样物质的生物合成和释放，也不作用于PGE2受体。它们抑制PG样物质的生物合成和释放：TF比芦丁和异槲皮苷更具活性：TF的ID30为1.03±0.24毫克/毫升，而芦丁和异槲皮苷分别为3.75±0.24毫克/毫升和2.31±1.4毫克/毫升。