Biochemical Research Center, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Biochemical Research Center, Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran; Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.
Eur J Pharm Sci. 2021 Dec 1;167:106031. doi: 10.1016/j.ejps.2021.106031. Epub 2021 Oct 1.
In this study, biodegradable and thermosensitive F127 hydrogel containing folic acid.MgO:ZnO/chitosan hybrid particles (FMZC) was fabricated as a 3D mesenchymal stem cells (MSCs) delivery vehicle for regenerative medicine and wound healing purposes, in such a way to be responsive to lysozyme and UVA irradiation. The results showed that F127 hydrogel containing FMZC is a suitable and nontoxic construct for encapsulation of MSCs in the presence of lysozyme and UVA irradiation, bearing high stem cell viability and proliferation. The final hydrogel, MSC&FMZC, in response to lysozyme induced a higher proliferation rate and migration in human foreskin fibroblast cells (HFF). These phenomena were attributed to the released F.MgO:ZnO nanocomposites from chitosan microparticles and paracrine factors from MSCs within the hydrogel, resulting in synergistic biological effects. Moreover, lysozyme-treated MSC&FMZC hydrogel showed higher antibacterial and anti-biofilm activity against both Gram-positive and Gram-negative bacteria than bare hydrogel. However, a significant increase in the antibacterial activity of MSC&FMZC was observed as the treated bacteria were subjected to UVA irradiation owing to the photocatalytic activity of F.MgO:ZnO nanocomposites. Regarding the antibacterial activity and stimulating skin cell behavior of MSC&FMZC hydrogel that can promote the regenerative activities of skin, it could be considered as a promising scaffold for bacteria-accompanied wound healing.
在这项研究中，制备了一种含有叶酸的可生物降解和温敏性 F127 水凝胶，其中包含 MgO:ZnO/壳聚糖杂化颗粒（FMZC），用作 3D 间充质干细胞（MSCs）的输送载体，用于再生医学和伤口愈合目的，以响应溶菌酶和 UVA 照射。结果表明，含有 FMZC 的 F127 水凝胶是一种适合且无毒的构建体，可在存在溶菌酶和 UVA 照射的情况下封装 MSCs，具有高干细胞活力和增殖率。最终的水凝胶 MSC&FMZC 对溶菌酶诱导的人包皮成纤维细胞（HFF）中的更高增殖率和迁移率做出响应。这些现象归因于壳聚糖微球中释放的 F.MgO:ZnO 纳米复合材料和水凝胶内 MSCs 的旁分泌因子，从而产生协同的生物学效应。此外，与裸水凝胶相比，经溶菌酶处理的 MSC&FMZC 水凝胶对革兰氏阳性菌和革兰氏阴性菌均表现出更高的抗菌和抗生物膜活性。然而，由于 F.MgO:ZnO 纳米复合材料的光催化活性，当处理过的细菌接受 UVA 照射时，MSC&FMZC 的抗菌活性显著增加。鉴于 MSC&FMZC 水凝胶具有抗菌活性和刺激皮肤细胞行为的能力，它可以促进皮肤的再生活动，因此可以被认为是一种有前途的伴随细菌的伤口愈合支架。
